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Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience

机译:Glycoscience @ Synchrotron:同步辐射应用于结构糖科学

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摘要

Synchrotron radiation is the most versatile way to explore biological materials in different states: monocrystalline, polycrystalline, solution, colloids and multiscale architectures. Steady improvements in instrumentation have made synchrotrons the most flexible intense X-ray source. The wide range of applications of synchrotron radiation is commensurate with the structural diversity and complexity of the molecules and macromolecules that form the collection of substrates investigated by glycoscience. The present review illustrates how synchrotron-based experiments have contributed to our understanding in the field of structural glycobiology. Structural characterization of protein–carbohydrate interactions of the families of most glycan-interacting proteins (including glycosyl transferases and hydrolases, lectins, antibodies and GAG-binding proteins) are presented. Examples concerned with glycolipids and colloids are also covered as well as some dealing with the structures and multiscale architectures of polysaccharides. Insights into the kinetics of catalytic events observed in the crystalline state are also presented as well as some aspects of structure determination of protein in solution.
机译:同步辐射是探索处于不同状态的生物材料的最通用方法:单晶,多晶,溶液,胶体和多尺度结构。仪器的不断改进使同步加速器成为最灵活的强X射线源。同步加速器辐射的广泛应用与构成糖科学研究的底物集合的分子和大分子的结构多样性和复杂性相称。本综述说明了基于同步加速器的实验如何有助于我们对结构糖生物学领域的理解。介绍了大多数聚糖相互作用蛋白(包括糖基转移酶和水解酶,凝集素,抗体和GAG结合蛋白)家族的蛋白质与碳水化合物相互作用的结构表征。还涉及与糖脂和胶体有关的例子,以及涉及多糖的结构和多尺度结构的一些例子。还介绍了在结晶状态下观察到的催化事件动力学的见解,以及溶液中蛋白质结构确定的某些方面。

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