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首页> 美国卫生研究院文献>The Journal of Neuroscience >Optical Postsynaptic Measurement of Vesicle Release Rates for Hippocampal Synapses Undergoing Asynchronous Release during Train Stimulation
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Optical Postsynaptic Measurement of Vesicle Release Rates for Hippocampal Synapses Undergoing Asynchronous Release during Train Stimulation

机译：光学突触后测量的火车刺激过程中进行异步释放的海马突触的囊泡释放速率。

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Developing hippocampal neurons in microisland culture were found to undergo rapid depression of excitatory synaptic activity caused by consumption of their readily releasable pool (RRP) of vesicles in response to 20 Hz trains of stimulation. Associated with depression was a switch to an asynchronous release mode that maintained transmission at a high steady-state rate equivalent to ∼2.1 RRPs per second. We have applied postsynaptic Ca2+ imaging to directly monitor these asynchronous release events to estimate both the steady rate of transmitter release and the number of quanta within the RRP at individual hippocampal synapses. Based on the frequency of asynchronous release measured at individual synapses postsynaptically using Ca2+ imaging (5-17 sec after train stimulation) and with knowledge of the time course by which asynchronous release rates decay, we estimate that individual hippocampal synapses exhibit (in response to train stimulation) peak release rates of up to 21 quanta per second from an RRP that contains, on average, 10 quanta. Use-dependent block of evoked synaptic activity by MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d]cyclohepten-5,10-imine maleate] confirmed that synapses undergoing asynchronous release are not significantly different from the general population with regard to their composition of NMDA receptor and/or release probability. Given that high-frequency trains deplete the synapse of readily releasable quanta (and that these release rates can only be maintained for a few seconds), these high rates of asynchronous release likely reflect refilling of vesicles from a reserve pool and not necessarily the continuous action of a relatively slow clathrin- and endosome-dependent process.
机译：发现在小岛文化中发展中的海马神经元经历了对20 Hz刺激序列的消耗，由于消耗了其易释放的囊泡（RRP）引起的兴奋性突触活性快速下降。与抑郁症相关的是切换到异步释放模式，该模式将传输保持在每秒约2.1 RRP的高稳态速率下。我们已经应用了突触后Ca 2 + 成像来直接监视这些异步释放事件，以估计单个海马突触中发射器释放的稳定速率和RRP内的量子数量。根据使用Ca 2 + 成像（火车刺激后5-17秒）在突触后突触测量的异步释放频率，并了解异步释放速率衰减的时间过程，我们估计单个海马突触显示（响应于训练刺激）从平均包含10个量子点的RRP释放的峰值释放速率高达每秒21个量子点。 MK-801 [（+）-5-甲基-10,11-二氢-5H-二苯并[a，d] cyclohepten-5,10-亚胺马来酸酯]引起的使用依赖性突触活性阻滞证实了突触正在进行异步释放就其NMDA受体的组成和/或释放可能性而言，它们与一般人群没有显着差异。鉴于高频火车耗尽了容易释放的量子的突触（并且这些释放速率只能维持几秒钟），这些高的异步释放速率可能反映了储备池中囊泡的充盈，而不一定是连续作用网格蛋白和内体依赖过程相对缓慢。

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