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首页> 美国卫生研究院文献>Antioxidants >Comparative Study of Antioxidant Power Polyphenols Flavonoids and Betacyanins of the Peel and Pulp of Three Tunisian Opuntia Forms
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Comparative Study of Antioxidant Power Polyphenols Flavonoids and Betacyanins of the Peel and Pulp of Three Tunisian Opuntia Forms

机译：三种突尼斯仙人掌形果皮和果肉抗氧化能力多酚类黄酮和甜菜碱的比较研究

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摘要

The antioxidant activity and the chemical composition of methanol extracts from peel and pulp belonging to two species of Tunisian prickly pears Opuntia ficus indica (spiny and thornless forms) and Opuntia stricta have been studied. The antioxidant capacity was measured by DPPH radical scavenging activity. The total phenolic compound (TPC) and the total flavonoid content were determined by the Folin–Ciocalteu method and colorimetric method, respectively. The phenolic compounds were identified and quantified by high-performance liquid chromatography (HPLC) coupled with an electrospray ionization mass spectrometry (ESI-MS). The results showed that O. stricta fruits present the best antioxidant activities than the two forms of O. ficus indica, while the TPC was more important in O. ficus indica than in the O. stricta fruits. The peels have higher flavonoids than pulp, and the thornless variety has more flavonoid than the spiny. The RP-HPLC and ESI-MS analysis detected two classes of phenolic compounds and betalain pigments. Isorhamnetin derivatives are the dominant flavonol glycoside identified in O. ficus indica (spiny: 65.25 μg·g−1; thornless: 77.03 μg·g−1) and O. stricta peels (19.22 μg·g−1).
机译：研究了突尼斯仙人掌果（仙人掌和无刺形式）和严格仙人掌的两种树皮和果肉中甲醇提取物的抗氧化活性和化学成分。通过DPPH自由基清除活性来测量抗氧化能力。总酚化合物（TPC）和总黄酮含量分别通过Folin–Ciocalteu方法和比色法确定。通过高效液相色谱（HPLC）结合电喷雾电离质谱（ESI-MS）对酚类化合物进行鉴定和定量。结果表明，比起两种形式的印度曲霉，欧洲曲霉（O.stricta）果实表现出最好的抗氧化活性，而在印度曲霉（O.ficus indica）中，TPC更为重要。果皮的黄酮含量高于果肉，无刺品种的黄酮含量高于棘刺。 RP-HPLC和ESI-MS分析检测到两类酚类化合物和甜菜色素。异鼠李素衍生物是O稻中的主要黄酮糖苷（多刺：65.25μg·g -1 ；无刺：77.03μg·g -1 ）和O. stricta果皮（19.22μg·g -1 ）。

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