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首页> 美国卫生研究院文献>Asian-Australasian Journal of Animal Sciences >In vitro and Lactation Responses in Mid-lactating Dairy Cows Fed Protected Amino Acids and Fat
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In vitro and Lactation Responses in Mid-lactating Dairy Cows Fed Protected Amino Acids and Fat

机译：饲喂受保护的氨基酸和脂肪的中期泌乳奶牛的体外和泌乳反应

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The objective of this study was to evaluate the effect of ruminally protected amino acids (RPAAs) and ruminally protected fat (RPF) supplementation on ruminal fermentation characteristics (in vitro) and milk yield and milk composition (in vivo). Fourteen mid-lactating Holstein dairy cows (mean weight 653±62.59 kg) were divided into two groups according to mean milk yield and number of days of postpartum. The cows were then fed a basal diet during adaptation (2 wk) and experimental diets during the treatment period (6 wk). Dietary treatments were i) a basal diet (control) and ii) basal diet containing 50 g of RPAAs (lysine and methionine, 3:1 ratio) and 50 g of RPF. In rumen fermentation trail (in vitro), RPAAs and RPF supplementation had no influence on the ruminal pH, dry matter digestibility, total volatile fatty acid production and ammonia-N concentration. In feeding trial (in vivo), milk yield (p<0.001), 4% fat corrected milk (p<0.05), milk fat (p<0.05), milk protein (p<0.001), and milk urea nitrogen (p<0.05) were greater in cows fed RPAAs and RPF than the corresponding values in the control group. With an index against as 0%, the rates of decrease in milk yield and milk protein were lower in RPAAs and RPF treated diet than those of basal diet group (p<0.05). In conclusion, diet supplemented with RPAAs and RPF can improve milk yield and milk composition without negatively affecting ruminal functions in Holstein dairy cows at mid-lactating.
机译：这项研究的目的是评估反刍动物保护的氨基酸（RPAA）和反刍动物保护的脂肪（RPF）补充对瘤胃发酵特性（体外），产奶量和泌乳成分（体内）的影响。根据平均产奶量和产后天数，将14头中生荷斯坦奶牛（平均体重653±62.59 kg）分为两组。然后在适应期间（2周）给母牛喂基础饲料，在治疗期间（6周）给它们喂实验饲料。饮食治疗包括：i）基础饮食（对照）和ii）基础饮食，其中包含50克RPAA（赖氨酸和蛋氨酸，比例为3：1）和50克RPF。在瘤胃发酵试验中（体外），补充RPAA和RPF对瘤胃pH，干物质消化率，总挥发性脂肪酸产量和氨氮浓度没有影响。在喂养试验（体内）中，牛奶产量（p <0.001），4％脂肪校正的牛奶（p <0.05），牛奶脂肪（p <0.05），牛奶蛋白（p <0.001）和牛奶尿素氮（p <饲喂RPAA和RPF的母牛的0.05）高于对照组的相应值。相对于基础饮食组，RPAAs和RPF处理的饮食中牛奶产量和乳蛋白的降低率以0％的指数降低（p <0.05）。总之，日粮中补充有RPAA和RPF的饲料可以提高产奶量和乳成分，而不会对泌乳中期荷斯坦奶牛的瘤胃功能产生负面影响。

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