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首页> 美国卫生研究院文献>Asian-Australasian Journal of Animal Sciences >Effects of Saturated Long-chain Fatty Acid on mRNA Expression of Genes Associated with Milk Fat and Protein Biosynthesis in Bovine Mammary Epithelial Cells
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Effects of Saturated Long-chain Fatty Acid on mRNA Expression of Genes Associated with Milk Fat and Protein Biosynthesis in Bovine Mammary Epithelial Cells

机译：饱和长链脂肪酸对牛乳上皮细胞乳脂相关基因mRNA表达和蛋白质生物合成的影响

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This study was conducted to determine the effects of saturated long-chain fatty acids (LCFA) on cell proliferation and triacylglycerol (TAG) content, as well as mRNA expression of αs1-casein (CSN1S1) and genes associated with lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows, and were passaged twice. Then cells were cultured with different levels of palmitate or stearate (0, 200, 300, 400, 500, and 600 μM) for 48 h and fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L). The results showed that cell proliferation tended to be increased quadratically with increasing addition of stearate. Treatments with palmitate or stearate induced an increase in TAG contents at 0 to 600 μM in a concentration-dependent manner, and the addition of 600 μM was less effective in improving TAG accumulation. The expression of acetyl-coenzyme A carboxylase alpha, fatty acid synthase and fatty acid-binding protein 3 was inhibited when palmitate or stearate were added in culture medium, whereas cluster of differentiation 36 and CSN1S1 mRNA abundance was increased in a concentration-dependent manner. The mRNA expressions of peroxisome proliferator-activated receptor gamma, mammalian target of rapamycin and signal transducer and activator of transcription 5 with palmitate or stearate had no significant differences relative to the control. These results implied that certain concentrations of saturated LCFA could stimulate cell proliferation and the accumulation of TAG, whereas a reduction may occur with the addition of an overdose of saturated LCFA. Saturated LCFA could up-regulate CSN1S1 mRNA abundance, but further studies are necessary to elucidate the mechanism for regulating milk fat and protein synthesis.
机译：这项研究旨在确定饱和长链脂肪酸（LCFA）对细胞增殖和三酰基甘油（TAG）含量以及αs1-酪蛋白（CSN1S1）的mRNA表达以及与牛脂质和蛋白质合成相关的基因的影响乳腺上皮细胞（BMEC）。从荷斯坦奶牛的乳腺中分离出原代细胞，并传代两次。然后将细胞用不同水平的棕榈酸酯或硬脂酸酯（0、200、300、400、500和600μM）培养48小时，并将培养液中的胎牛血清替换为不含脂肪酸的BSA（1 g / L ）。结果表明，随着硬脂酸盐的添加，细胞增殖倾向于二次增加。棕榈酸酯或硬脂酸酯的处理以0到600μM的浓度依赖性诱导TAG含量的增加，而600μM的添加在改善TAG积累方面效果较差。当在培养基中加入棕榈酸酯或硬脂酸酯时，乙酰辅酶A羧化酶α，脂肪酸合酶和脂肪酸结合蛋白3的表达受到抑制，而分化簇36和CSN1S1 mRNA的丰度则以浓度依赖的方式增加。过氧化物酶体增殖物激活的受体γ，雷帕霉素的哺乳动物靶标以及棕榈酸酯或硬脂酸酯的信号转导和转录激活剂5的mRNA表达与对照相比无显着差异。这些结果表明，一定浓度的饱和LCFA可以刺激细胞增殖和TAG的积累，而过量添加饱和LCFA可能会导致细胞的减少。饱和的LCFA可以上调CSN1S1 mRNA的丰度，但是需要进一步的研究来阐明调节乳脂和蛋白质合成的机制。

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