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首页> 美国卫生研究院文献>Asian-Australasian Journal of Animal Sciences >Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene
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Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene

机译：基于部分线粒体16S rRNA基因的等位基因特异性聚合酶链反应快速确定北方毛aux虾（Acetes chinensis）的起源

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摘要

Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis′ economic value, however, is low in China, and much of the catch in China is exported to Korea at a low price, thus leading to potential false labeling. For this reason, we developed a simple method to identify A. chinensis′ origin using allele-specific polymerase chain reaction (PCR). Ten single nucleotide polymorphisms (SNPs) were identified from partial (i.e., 570 bp) DNA sequence analysis of the mitochondrial 16s rRNA gene in 96 Korean and 96 Chinese individual shrimp. Among 10 SNP sites, four sites were observed in populations from both countries, and two sites located in the middle with SNP sites at their 3′-ends were used to design allele-specific primers. Among the eight internal primers, the C220F primer specific to the Chinese A. chinensis population amplified a DNA fragment of 364 bp only from that population. We were able to identify the A. chinensis population origin with 100% accuracy using multiplex PCR performed with two external primers and C220F primers. These results show that the 16S rRNA gene that is generally used for the identification of species can be used for the identification of the origin within species of A. chinensis, which is an important finding for the fair trade of the species between Korea and China.
机译：中华绒螯蟹（Acetes chinensis）是一种经济上重要的虾，属于虾Ser科。然而，发酵后，中国的中华曲霉的经济价值很低，中国的许多渔获物以低价出口到韩国，从而导致潜在的虚假标签。因此，我们开发了一种使用等位基因特异性聚合酶链反应（PCR）识别中华曲霉起源的简单方法。通过对96只韩国虾和96只中国虾的线粒体16s rRNA基因进行部分（即570 bp）DNA序列分析，鉴定出10个单核苷酸多态性（SNP）。在这两个国家的10个SNP位点中，在两个国家的人口中均观察到了4个位点，并且使用位于中间3个末端带有SNP位点的两个位点设计等位基因特异性引物。在八种内部引物中，中国中华曲霉种群的特异性C220F引物仅从该种群扩增出364 bp的DNA片段。使用两个外部引物和C220F引物进行的多重PCR，我们能够以100％的准确性鉴定中华曲霉的种群起源。这些结果表明，通常用于鉴定物种的16S rRNA基因可以用于鉴定中华曲霉物种内的起源，这对于中韩之间的物种公平交易是一个重要发现。

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