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首页> 美国卫生研究院文献>Asian Journal of Andrology >Genetic polymorphisms in glutathione S-transferase T1 affect the surgical outcome of varicocelectomies in infertile patients
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Genetic polymorphisms in glutathione S-transferase T1 affect the surgical outcome of varicocelectomies in infertile patients

机译：谷胱甘肽S-转移酶T1基因多态性影响不育患者精索静脉曲张手术的疗效

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Glutathione S-transferases (GSTs), superoxide dismutase 2 (SOD2) and NAD(P)H:quinone oxidoreductase 1 (NQO1) are anti-oxidant enzyme genes. Polymorphisms of GSTs, SOD2 and NQO1 have been reported to influence individual susceptibility to various diseases. In an earlier study, we obtained preliminary findings that a subset of glutathione S-transferase T1 (GSTT1)-wt patients with varicocele may exhibit good response to varicocelectomy. In this study, we extended the earlier study to determine the distribution of genotype of each gene in the infertile population and to evaluate whether polymorphism of these genes affects the results of surgical treatment of varicocele. We analyzed 72 infertile varicocele patients, 202 infertile patients without varicocele and 101 male controls. Genotypes of GSTs were determined by polymerase chain reaction (PCR). Genotyping of SOD2 and NQO1 was performed using the PCR-restriction fragment length polymorphism (PCR-RFLP) method. A significantly better response to varicocelectomy was found in patients with the GSTT1-wt genotype (63.2%) and NQO1-Ser/Ser genotype (80.0%) than in those with GSTT1-null genotype (35.3%) and NQO1-Pro/Pro or NQO1-Pro/Ser genotype (45.2%), respectively. The frequencies of glutathione S-transferase M1/T1, SOD2 and NQO1 genotypes did not differ significantly among the varicocele patients, idiopathic infertile patients and male controls. GSTT1 genotype is associated with improvement of semen parameters after varicocelectomy. As the number of patients with NQO1-Ser/Ser genotype was not sufficient to reach definite conclusions, the association of NQO1 genotype with varicocelectomy requires further investigation.
机译：谷胱甘肽S-转移酶（GSTs），超氧化物歧化酶2（SOD2）和NAD（P）H：醌氧化还原酶1（NQO1）是抗氧化酶基因。据报道，GST，SOD2和NQO1的多态性会影响个体对各种疾病的易感性。在较早的研究中，我们获得了初步发现，即精索静脉曲张的一部分谷胱甘肽S-转移酶T1（GSTT1）-wt患者可能对精索静脉曲张切除术表现出良好的反应。在这项研究中，我们扩展了先前的研究，以确定每个基因在不育人群中的基因型分布，并评估这些基因的多态性是否会影响精索静脉曲张的手术治疗结果。我们分析了72例不育精索静脉曲张患者，202例无精索静脉曲张不育患者和101例男性对照。 GST的基因型通过聚合酶链反应（PCR）确定。使用PCR限制性片段长度多态性（PCR-RFLP）方法对SOD2和NQO1进行基因分型。 GSTT1-wt基因型（63.2％）和NQO1-Ser / Ser基因型（80.0％）的患者对精索静脉曲张切除的反应明显好于GSTT1-null基因型（35.3％）和NQO1-Pro / Pro或 NQO1 -Pro / Ser基因型（45.2％）。精索静脉曲张中谷胱甘肽 S 转移酶 M1 / T1 ， SOD2 和 NQO1 的频率没有显着差异患者，特发性不育患者和男性对照者。 GSTT1 基因型与精索静脉曲张切除术后精液参数的改善有关。由于 NQO1 -Ser / Ser基因型的患者人数不足以得出明确的结论， NQO1 基因型与精索静脉曲张切除术的关联需要进一步研究。

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