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Production of δ-Endotoxin by Bacillus thuringiensis as a Function of Glucose Concentrations

机译:苏云金芽孢杆菌产生δ-内毒素与葡萄糖浓度的关系

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摘要

The insect pathogen Bacillus thuringiensis was cultured at different glucose concentrations. Size, protein content, and insecticidal activity of the parasporal inclusions, which are formed during the sporulation process, were measured. Increased glucose concentrations led to bigger crystalline inclusions with a higher content of protein and insecticidal activity. Maximum yields of protein and endotoxin were obtained in a semisynthetic medium that contained glucose concentrations of 6 to 8 g/liter.
机译:在不同的葡萄糖浓度下培养昆虫病原体苏云金芽孢杆菌。测量了在孢子形成过程中形成的孢子旁夹杂物的大小,蛋白质含量和杀虫活性。葡萄糖浓度增加导致更大的晶体夹杂物,具有更高的蛋白质含量和杀虫活性。在含有6至8 g / L葡萄糖浓度的半合成培养基中可获得最大的蛋白质和内毒素产量。

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