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Acetylene Reduction (Nitrogen Fixation) by Pulp and Paper Mill Effluents and by Klebsiella Isolated from Effluents and Environmental Situations

机译:纸浆和造纸厂废水以及从废水和环境中分离出的克雷伯菌减少的乙炔(固氮)

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摘要

High rates of acetylene (C2H2) reduction (nitrogenase activity) were observed in woodroom effluent from a neutral sulfite semi-chemical mill under aerobic (up to 644 nmol of C2H4 produced per ml per h) and under anaerobic (up to 135 nmol of C2H4 produced per ml per h) conditions. Pasteurized effluent developed C2H2 reduction activity when incubated under anaerobic but not under aerobic conditions. Activities were increased by addition of 0.5 to 3.0% glucose or xylose. Enrichment and enumeration studies showed that N2-fixing Azotobacter and Klebsiella were abundant, and N2-fixing Bacillus was present. Of 129 isolates of Klebsiella from pulp mills, lakes, rivers, and drainage and sewage systems, 32% possessed nitrogen-fixing ability.
机译:在中性亚硫酸盐半化学工厂在有氧条件下(每毫升每小时最多产生644 nmol的C2H4)和厌氧条件下(最高135 nmol的C2H4),在中性亚硫酸盐半化学工厂的木房废水中观察到乙炔(C2H2)的高还原率(氮酶活性)。在每毫升每小时(h)的条件下产生。当在厌氧条件下而不是在需氧条件下温育时,巴氏杀菌的废水具有降低C2H2的活性。通过添加0.5%至3.0%的葡萄糖或木糖可以增加活性。富集和计数研究表明,固定有N2的固氮杆菌和克雷伯菌属丰富,并且存在固定有N2的芽孢杆菌。来自制浆厂,湖泊,河流,排水系统和污水系统的129株克雷伯菌分离株,其中32%具有固氮能力。

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