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Rapid detection of bacterial endotoxins in drinking water and renovated wastewater.

机译:快速检测饮用水和更新后的废水中的细菌内毒素。

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摘要

A pilot study was conducted to determine the feasibility of using the Limulus endotoxin assay to detect endotoxins in potable waters and from reclaimed advanced waste treatment (AWT) plant effluents. Water samples were tested using both Limulus lysates prepared in our laboratory and a commercial product, Difco Pyrotest. The Limulus assay procedure was easily adapted to the testing of water samples for endotoxin. Measured endotoxin concentrations varied from 0.78 ng/ml to 1,250 ng/ml. Levels of endotoxin were not predictable based on whether the water was drinking water or AWT water, i.e., some AWT water samples had less endotoxin activity than some samples of drinking water, and some AWT waters had greater endotoxin activity than drinking water. Only three of the water samples tested were free of any detectable endotoxin. Breakpoint chlorination procedures seemed to reduce measurable endotoxin content, whereas passage through activated carbon columns was associated with greater final endotoxin concentrations in test waters.
机译:进行了一项初步研究,以确定使用the内毒素测定法来检测饮用水和再生高级废物处理(AWT)植物废水中的内毒素的可行性。使用在我们实验室中制备的Li裂解物和商业产品Difco Pyrotest测试水样品。 mul测定程序很容易适应于测试水样中的内毒素。测得的内毒素浓度从0.78 ng / ml到1,250 ng / ml不等。根据水是饮用水还是AWT水,无法预测内毒素的水平,即某些AWT水样品的内毒素活性低于某些饮用水样品,某些AWT水的内毒素活性高于饮用水。测试的水样本中只有三个不含任何可检测到的内毒素。断点氯化程序似乎减少了可测量的内毒素含量,而通过活性炭柱则与测试水中较高的最终内毒素浓度有关。

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