Preservation of gastrointestinal bacteria and their microenvironmental associations in rats by freezing.

机译：冷冻保存大鼠胃肠道细菌及其微环境联系。

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The use of frozen rat gastrointestinal tissue samples for both the recovery of viable bacteria and for observation of microbial communities associated with the tissue was investigated. A decrease of 1 log in lactobacilli, bifidobacteria, and anaerobes was observed when the numbers of bacteria recoverable from frozen tissue (stored 7 to 9 days) were compared to those recoverable from fresh nonfrozen tissue (zero time control). However, freezing did not appear to decrease the numbers of recoverable coliforms. Tissues, cleaved with razor blades after being frozen and stored for 7 to 9 days, showed bacterial communities situated on the mucosa and in the lumen of gastrointestinal specimens. This freezing technique preserved structures not previously observed in the gastrointestinal tract. This indicates that freezing is a good method to use to study such fragile microenvironments.

机译：研究了冷冻大鼠胃肠组织样品在活细菌的回收以及与组织相关的微生物群落的观察中的用途。当将可从冷冻组织（储存7至9天）中回收的细菌数量与可从新鲜非冷冻组织中回收的细菌数量（零时间对照）进行比较时，观察到乳酸杆菌，双歧杆菌和厌氧菌减少了1 log。但是，冷冻似乎并未减少可回收大肠菌的数量。冷冻并保存7至9天后用剃须刀劈开的组织显示出位于粘膜和胃肠道标本腔内的细菌群落。这种冷冻技术保留了以前在胃肠道中未观察到的结构。这表明冷冻是研究这种脆弱的微环境的好方法。

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期刊名称 Applied and Environmental Microbiology
作者
C P Davis;
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作者单位

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年(卷),期 1976(31),2
年度 1976
页码 304–312
总页数 9
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
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专利

1. Preservation of gastrointestinal bacteria and their microenvironmental associations in rats by freezing. [J] . C P Davis Applied and Environmental Microbiology . 1976,第2期

机译：冷冻保存大鼠胃肠道细菌及其微环境联系。
2. Combined strategy for fertility preservation in an oncologic patient: vitrification of in vitro matured oocytes and ovarian tissue freezing. [J] . Gonzalez C, Devesa M, Boada M, Journal of assisted reproduction and genetics . 2011,第12期

机译：肿瘤患者保留生育力的联合策略：体外成熟卵母细胞玻璃化和卵巢组织冷冻。
3. Interleukin-6 and tumor necrosis factor-alpha levels following hepatic cryotherapy: association with volume and duration of freezing. [J] . Seifert JK, Stewart GJ, Hewitt PM, World Journal of Surgery: Official Journal of the Societe Internationale de Chirurgie, Collegium Internationale Chirurgiae Digestivae, and of the International Association of Endocrine Surgeons . 1999,第10期

机译：肝冷冻治疗后白细胞介素6和肿瘤坏死因子-α水平：与冷冻量和持续时间相关。
4. Association rule sharing model for privacy preservation and collaborative data mining efficiency [C] . KumaraSwamy S, Manjula S H, Venugopal K R, 2014 Recent Advances in Engineering and Computational Sciences . 2014

机译：用于隐私保护和协作数据挖掘效率的关联规则共享模型
5. The association between plasma 25-hydroxyvitamin D concentration and oral bacteria among postmenopausal women. [D] . Sahli, Michelle W. 2012

机译：绝经后妇女血浆25-羟基维生素D浓度与口腔细菌之间的关系。
6. Self-preservation strategies during bacterial biomineralization with reference to hydrozincite and implications for fossilization of bacteria [O] . Bryne T. Ngwenya, Marisa Magennis, Francesca Podda, 2014

机译：关于水合锌矿的细菌生物矿化过程中的自我保存策略及其对细菌化石的影响
7. Figure 5: Colorimetric measurements—after room temperature preservation, isobaric freezing and isochoric freezing. [O] . -1

机译：图5：比色测量 - 室温保存，阻碍冻结和等离冻结。
8. Simulated Silicification of Bacteria - New Clues to the Modes and Timing of Bacterial Preservation and Implications for the Search for Extraterrestrial Microfossils [R] . 2002

机译：细菌的模拟硅化 - 细菌保存模式和时间的新线索及对寻找外星微化石的启示

Preservation of gastrointestinal bacteria and their microenvironmental associations in rats by freezing.

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