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Virus accumulation by the rock oyster Crassostrea glomerata.

机译:牡蛎Crassostrea glomerata堆积病毒。

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摘要

The accumulation of virus by the New Zealand rock oyster Crassostrea glomerata has been studied in a static seawater system using radioactively labeled reovirus type III and Semliki Forest virus. The uptake of virus was found to be less rapid than for the bacterium Escherichia coli and to be unaffected by the presence of the marine alga Dunaliella primolecta in the seawater. Accumulation was dependent on virus concentration, with saturation achieved at 4 X 10(10) reovirus particles per oyster, implying that an oyster possesses a large but finite number of sites for virus adsorption. When the rates of uptake of two viruses of similar size but differing surface properties were compared, the rate of accumulation of the lipoprotein-enveloped Semliki Forest virus was found to be less than that for the protein-enclosed reovirus. This observation, together with the finding that the oyster shell has a strong affinity for virus, suggests that surface properties, rather than size, are the principal factors governing the accumulation of viruses by filter-feeding marine bivalves.
机译:已使用放射性标记的III型呼肠孤病毒和Semliki森林病毒在静态海水系统中研究了新西兰牡蛎Crassostrea glomerata积累的病毒。发现病毒的吸收不如大肠杆菌快,并且不受海水中杜氏杜氏藻的存在的影响。积累取决于病毒的浓度,每个牡蛎在4 X 10(10)呼肠孤病毒颗粒中达到饱和,这意味着牡蛎拥有大量但有限的病毒吸附位点。当比较两种大小相似但表面特性不同的病毒的摄取速率时,发现脂蛋白包裹的Semliki Forest病毒的积累速率低于蛋白质包裹的呼肠孤病毒。该观察结果以及牡蛎壳对病毒具有很强的亲和力的发现表明,表面特性而非大小是通过滤食海洋双壳类动物控制病毒积累的主要因素。

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