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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >High-pressure-temperature gradient instrument: use for determining the temperature and pressure limits of bacterial growth.
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High-pressure-temperature gradient instrument: use for determining the temperature and pressure limits of bacterial growth.

机译：高压温度梯度仪：用于确定细菌生长的温度和压力极限。

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摘要

A pressurized temperature gradient instrument allowed a synoptic determination of the effects of temperature and pressure on the reproduction of bacteria. The instrument consisted of eight pressure vessels housed parallel to each other in an insulated aluminum block in which a linear temperature gradient was supported. For a given experiment, eight pressures between 1 and 1,100 bars were chosen; the linear temperature gradient was established over an interval within -20 to 100 degrees C. Pure cultures and natural populations were studied in liquid or solid medium either in short (ca. 2-cm) culture tubes or in long (76.2-cm) glass capillaries. In the case of a pure culture, experiments with the pressurized temperature gradient instrument determined values of temperature and pressure that bounded its growth. Feasibility experiments with mixed populations of bacteria from water samples from a shallow depth of the sea showed that the instrument may be useful in identifying the extent to which a natural population is adapted to the temperatures and pressures at the locale of origin of the sample. Additional conceived uses of the instrument included synoptic determinations of cell functions other than reproduction and of biochemical activities.
机译：加压的温度梯度仪器可以对温度和压力对细菌繁殖的影响进行概要分析。该仪器由八个平行放置在绝缘铝块中的压力容器组成，铝块中支撑着线性温度梯度。对于给定的实验，选择了1至1100巴之间的8个压力。在-20至100摄氏度之间的时间间隔内建立了线性温度梯度。在短（约2厘米）培养管或长（76.2厘米）培养皿中的液体或固体培养基中研究纯培养物和自然种群毛细管。对于纯培养物，使用加压温度梯度仪进行的实验确定了限制其生长的温度和压力值。对来自浅海深处的水样本中细菌的混合种群进行的可行性实验表明，该仪器可用于确定自然种群适应样本来源区域的温度和压力的程度。该仪器的其他设想用途包括概要确定除繁殖和生化活性以外的细胞功能。

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