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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Bacterial Biomass Metabolic State and Activity in Stream Sediments: Relation to Environmental Variables and Multiple Assay Comparisons
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Bacterial Biomass Metabolic State and Activity in Stream Sediments: Relation to Environmental Variables and Multiple Assay Comparisons

机译：河流沉积物中的细菌生物量代谢状态和活性：与环境变量的关系和多重分析的比较

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Bacterial biomass, metabolic condition, and activity were measured over a 16-month period in the surface sediments of the following four field sites with differing dissolved organic matter regimes: a woodlot spring seep, a meadow spring seep, a second-order stream, and a third-order stream. Total bacterial biomass was measured by lipid phosphate and epifluorescence microscopic counts (EMC), and viable biomass was measured by 14C most probable number, EMC with 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride reduction, and ATP. Bacterial metabolic condition was determined from the percentage of respiring cells, poly-β-hydroxybutyrate concentrations, and adenylate energy charge. Activity measures included 14C-lipid synthesis, 32P-phospholipid synthesis, the rate of uptake of algal lysate dissolved organic carbon, and respiration, from which biosynthesis was calculated (dissolved organic carbon uptake corrected for respiration). Total bacterial biomass (from EMC) ranged from 0.012 to 0.354 μg of C/mg of dry sediment and was usually lowest in the third-order stream. The percentage of cells respiring was less than 25% at all sites, indicating that most bacteria were dormant or dead. Adenylate energy charge was measured only in the third-order stream and was uniformly low. Poly-β-hydroxybutyrate concentrations were greater in the woodlot spring seep than in the second- and third-order streams. Uptake of algal lysate dissolved organic carbon ranged from undetectable levels to 166 mg of C · m−2 · h−1. Little community respiration could be attributed to algal lysate metabolism. Phospholipid synthesis ranged from 0.006 to 0.354 pmol · mg of dry sediment−1 · h−1. Phospholipid synthesis rates were used to estimate bacterial turnover at the study sites. An estimated 375 bacterial generations per year were produced in the woodlot spring seep, and 67 per year were produced in the third-order stream.
机译：在以下四个田间具有不同溶解有机质状况的田间地表沉积物中，在16个月的时间内测量了细菌生物量，代谢状况和活性：林地泉水，草甸泉水，二阶水流和三阶流。用脂质磷酸酯和表观荧光显微镜计数法（EMC）测定细菌总生物量，用 14 C最可能数EMC和2-（对-碘苯基）-3-（p-硝基苯基）-5-苯基氯化四氮唑还原和ATP。根据呼吸细胞的百分比，聚-β-羟基丁酸酯浓度和腺苷酸能电荷确定细菌代谢状况。活性测量包括 14 C脂质合成， 32 P磷脂合成，藻类裂解物溶解的有机碳的吸收速率和呼吸，由此可计算生物合成（溶解校正了呼吸的有机碳吸收）。总细菌生物量（来自EMC）范围为0.012至0.354μgC / mg干沉积物，通常在三级流中最低。在所有部位，细胞呼吸的百分比均低于25％，表明大多数细菌处于休眠或死亡状态。腺苷酸的能量电荷仅在三阶流中测量，并且始终较低。林地春季渗水中的聚-β-羟基丁酸酯浓度高于二阶和三阶物流。藻类溶解产物溶解的有机碳的吸收范围为无法检测到的166 mg C·m -2 ·h -1 。很少的社区呼吸作用可归因于藻类裂解物的新陈代谢。磷脂的合成范围为0.006至0.354 pmol·干沉积物 -1 ·h -1 mg。磷脂合成速率用于估计研究部位的细菌更新。在林地的春季渗水中，每年估计产生375个细菌，而在三阶水流中，每年产生67个细菌。

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