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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Dynamics of Soil Denitrifier Populations: Relationships between Enzyme Activity Most-Probable-Number Counts and Actual N Gas Loss
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Dynamics of Soil Denitrifier Populations: Relationships between Enzyme Activity Most-Probable-Number Counts and Actual N Gas Loss

机译：土壤反硝化菌种群的动态：酶活性最有可能发生的数量计数和实际N气体损失之间的关系

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摘要

To better understand temporal variability in soil denitrification, denitrifying enzyme activity (DEA) and denitrifier populations (as determined by most-probable-number [MPN] counts) were measured in field and laboratory experiments. Measurements of DEA and MPN provided highly contradictory indications of denitrifier dynamics. In laboratory incubations, under conditions favoring active denitrification, the synthesis of new denitrifying enzymes and the actual amount of denitrification were closely related. In other experiments, however, both DEA and MPN counts were poor indicators of actual denitrification. In some cases, we found significant increases in DEA but no significant production of N gas. Except with unnaturally high substrate amendments, changes in DEA were small relative both to the persistently high DEA background and to changes in MPN. As estimated by MPN counts, denitrifier populations increased significantly during denitrification events. It was apparent that only a small fraction of the denitrifiers were included in the MPN counts, but it appeared that this isolatable fraction increased during periods of active denitrifier growth. Use of DEA as an index of biomass of cells which have synthesized denitrifying enzymes suggested that denitrifier populations were persistent, stable, and much larger than indicated by MPN procedures.
机译：为了更好地了解土壤反硝化作用的时间变异性，在野外和实验室实验中对反硝化酶活性（DEA）和反硝化菌种群（由最可能数[MPN]计数确定）进行了测量。 DEA和MPN的测量提供了反硝化动力学的高度矛盾的指示。在实验室温育中，在有利于主动反硝化的条件下，新反硝化酶的合成与反硝化的实际量密切相关。但是，在其他实验中，DEA和MPN计数都不是实际反硝化的指标。在某些情况下，我们发现DEA显着增加，但没有大量N气产生。除了底物修正值异常高外，相对于持续较高的DEA背景和MPN的变化，DEA的变化都很小。根据MPN计数估算，反硝化过程中反硝化剂的数量显着增加。显然，MPN计数中仅包含一小部分反硝化剂，但是在可活动的反硝化剂生长期间，该可分离部分似乎有所增加。使用DEA作为已合成反硝化酶的细胞生物量的指标表明，反硝化剂群体具有持久性，稳定性，并且比MPN程序指示的要大得多。

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