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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery
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Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery

机译：砂岩岩心中营养的恢复和饥饿细胞的生长：提高采油率的新方法

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摘要

Klebsiella pneumoniae, which was reduced in size (0.25 by 0.5 μm) by carbon deprivation, was injected into a series of sandstone cores and subjected to separate treatments. Scanning electron microscopy of 400-mD cores showed these small starved cells in nearly every core section. The cells were a mixture of small rods and cocci with little or no biofilm production. Continuous or dose stimulation with sodium citrate allowed the cells to grow throughout the sandstone and completely plug the length of the core. The resuscitated cells were larger than the starved cells (up to 1.7 μm) and were encased in glycocalyx. Scanning electron microscopic results of resuscitation in situ with half-strength brain heart infusion broth showed that a shallow “skin” plug of cells formed at the core inlet and that fewer cells were located in the lower sections. Starved cells also penetrated 200-mD cores and were successfully resuscitated in situ with sodium citrate, so that the entire core was plugged. Nutrient resuscitation of injected starved cells to produce full-size cells which grow and block the rock pores may be successfully applied to selective plugging and may effectively increase oil recovery.
机译：通过碳剥夺将肺炎克雷伯菌（Klebsiella pneumoniae）的大小减小（0.25毫米，减小0.5微米），将其注入一系列砂岩岩心中并进行单独处理。扫描电镜对400-mD核的观察表明，几乎每个核部分都存在这些小的饥饿细胞。细胞是小杆和球菌的混合物，几乎没有或没有生物膜产生。用柠檬酸钠连续或剂量刺激可使细胞在整个砂岩中生长并完全堵塞岩心的长度。复苏的细胞大于饥饿的细胞（最大1.7μm），并被包裹在糖萼中。用半强度脑灌注液原位复苏的扫描电子显微镜结果表明，在核心入口处形成了浅的“皮肤”细胞栓塞，而位于下部的细胞较少。饥饿的细胞也穿透了200 mD的核心，并成功地用柠檬酸钠原位复苏，从而堵塞了整个核心。对注入的饥饿细胞进行营养复苏以产生生长并阻塞岩石孔隙的全尺寸细胞，可以成功地应用于选择性封堵，并可以有效地提高采油率。

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