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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Presence of the fish pathogen Vibrio salmonicida in fish farm sediments.
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Presence of the fish pathogen Vibrio salmonicida in fish farm sediments.

机译：鱼类养殖场沉积物中存在鱼类病原体鲑鱼弧菌。

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摘要

The persistence of the fish pathogen Vibrio salmonicida in fish farm sediments was studied by use of fluorescent-antibody techniques. The specificities of the monoclonal antibodies and polyclonal rabbit serum used in the study were tested against a number of Vibrio strains, including 4 isolates from intestinal tracts of healthy fish and 98 isolates from sediments. V. salmonicida was detected in sediment samples from diseased farms several months after an outbreak of the disease. The bacterium was also detected in a sediment sample from a disease-free fish farm. No V. salmonicida could be detected in sediments not influenced by fish farming. The number of positive samples was generally higher with application of rabbit serum as opposed to use of monoclonal antibodies, indicating that the rabbit serum may cross-react with other bacteria.
机译：利用荧光抗体技术研究了鱼类病原体鲑鱼弧菌在鱼类养殖场沉积物中的持久性。针对多种弧菌菌株测试了用于该研究的单克隆抗体和多克隆兔血清的特异性，其中包括来自健康鱼类肠道的4种分离株和来自沉积物的98种分离株。疾病暴发几个月后，在患病农场的沉积物样本中发现了鲑鱼弧菌。还从无病养鱼场的沉积物样本中检测到了这种细菌。不受鱼类养殖影响的沉积物中未检测到鲑鱼弧菌。与使用单克隆抗体相反，使用兔血清时阳性样品的数量通常较高，这表明兔血清可能与其他细菌发生交叉反应。

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