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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Adhesive Properties of a Symbiotic Bacterium from a Wood-Boring Marine Shipworm
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Adhesive Properties of a Symbiotic Bacterium from a Wood-Boring Marine Shipworm

机译：木柴船生船虫共生细菌的黏附特性。

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摘要

Adhesive properties of a cellulolytic, nitrogen-fixing bacterium isolated from a marine shipworm by Waterbury et al. (J. B. Waterbury, C. B. Calloway, and R. D. Turner, Science 221:1401-1403, 1983) are described. 35S-labeled cells of the shipworm bacterium bound preferentially to Whatman no. 1 cellulose filter paper, compared with its binding to other cellulose substrata or substrata lacking cellulose. The ability of the bacteria to bind to Whatman no. 1 filter paper was significantly reduced by glutaraldehyde or heat treatment of cells. Pretreatment of cells with azide, valinomycin, gramicidin-D, bis-hexafluoroacetylacetone (1799), or carbonyl cyanide-p-trifluoromethoxyphenylhydrazone inhibited adhesion activity. Cells pretreated with pronase or trypsin also exhibited reduced binding activity, but chymotrypsin and peptidase had no effect on adhesion activity. Cellodextrins and methyl cellulose 15 inhibited the adhesion of shipworm bacteria to filter paper, whereas glucose, cellobiose, and soluble carboxymethyl cellulose had no significant effect. The divalent cation chelators EDTA and EGTA [ethylene glycol-bis(β-aminoethyl ether)-N,N,N′N′-tetraacetic acid] had little or no effect on adhesive properties of shipworm bacteria. Also, preabsorbing the substratum with extracellular endoglucanase isolated from the shipworm bacterium or 1% bovine serum albumin had no apparent effect on bacterial binding. Low concentrations (0.01%) of sodium dodecyl sulfate solubilized a fraction from whole cells, which appeared to be involved in cellular binding activity. After removal of sodium dodecyl sulfate, several proteins in this fraction associated with intact cells. These cells exhibited up to 50% enhanced binding to filter paper in comparison to cells which had not been exposed to the sodium dodecyl sulfate-solubilized fraction.
机译：Waterbury等人从海洋Water虫中分离出的纤维素分解固氮细菌的粘附特性。 （J.B.Waterbury，C.B.Calloway和R.D.Turner，Science 221：1401-1403，1983）被描述。 s虫细菌的 35 S标记细胞优先结合Whatman no。 1纤维素滤纸，它与其他纤维素基质或缺乏纤维素的基质结合的能力相比。细菌结合Whatman No的能力。 1张滤纸因戊二醛或细胞热处理而明显减少。用叠氮化物，缬氨霉素，短杆菌肽-D，双六氟乙酰丙酮（1799）或羰基氰化物-对-三氟甲氧基苯基hydr预处理细胞会抑制粘附活性。用链霉蛋白酶或胰蛋白酶预处理的细胞也表现出降低的结合活性，但是胰凝乳蛋白酶和肽酶对粘附活性没有影响。纤维糊精和甲基纤维素15抑制了ship虫细菌对滤纸的粘附，而葡萄糖，纤维二糖和可溶性羧甲基纤维素则没有显着影响。二价阳离子螯合剂EDTA和EGTA [乙二醇-双（β-氨基乙基醚）-N，N，N'N'-四乙酸]对]虫细菌的粘附性能影响很小或没有影响。另外，用从the虫细菌中分离的细胞外内切葡聚糖酶或1％牛血清白蛋白预吸收基质对细菌结合没有明显影响。低浓度（0.01％）的十二烷基硫酸钠可溶解全细胞中的一部分，似乎与细胞结合活性有关。除去十二烷基硫酸钠后，该级分中的几种蛋白质与完整细胞相关。与未暴露于十二烷基硫酸钠可溶级分的细胞相比，这些细胞与滤纸的结合提高了50％。

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