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Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures.

机译:通过反硝化富集培养物降解甲苯和间二甲苯并转化邻二甲苯。

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摘要

Seven different sources of inocula that included sediments, contaminated soils, groundwater, process effluent, and sludge were used to establish enrichment cultures of denitrifying bacteria on benzene, toluene, and xylenes in the absence of molecular oxygen. All of the enrichment cultures demonstrated complete depletion of toluene and partial depletion of o-xylene within 3 months of incubation. The depletion of o-xylene was correlated to and dependent on the metabolism of toluene. No losses of benzene, p-xylene, or m-xylene were observed in these initial enrichment cultures. However, m-xylene was degraded by a subculture that was incubated on m-xylene alone. Complete carbon, nitrogen, and electron balances were determined for the degradation of toluene and m-xylene. These balances showed that these compounds were mineralized with greater than 50% conversion to CO2 and significant assimilation into biomass. Additionally, the oxidation of these compounds was shown to be dependent on nitrate reduction and denitrification. These microbial degradative capabilities appear to be widespread, since the widely varied inoculum sources all yielded similar results.
机译:接种物的七种不同来源,包括沉积物,受污染的土壤,地下水,工艺废水和污泥,用于在分子氧不存在的情况下在苯,甲苯和二甲苯上建立反硝化细菌的富集培养。在孵育的3个月内,所有的富集培养物均显示出甲苯的完全消耗和邻二甲苯的部分消耗。邻二甲苯的消耗与甲苯的代谢有关,并取决于甲苯的代谢。在这些最初的富集培养中未观察到苯,对二甲苯或间二甲苯的损失。然而,间二甲苯通过仅在间二甲苯上孵育的传代培养而降解。确定了甲苯,间二甲苯降解的完全碳,氮和电子平衡。这些平衡表明,这些化合物已矿化,转化成CO2的比例超过50%,并且被大量吸收为生物质。另外,显示这些化合物的氧化取决于硝酸盐的还原和反硝化。这些微生物的降解能力似乎很普遍,因为各种各样的接种源均产生了相似的结果。

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