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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Isolation of Pseudomonas pickettii strains that degrade 246-trichlorophenol and their dechlorination of chlorophenols.
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Isolation of Pseudomonas pickettii strains that degrade 246-trichlorophenol and their dechlorination of chlorophenols.

机译：降解246-三氯苯酚的Pseudomonas pickettii菌株的分离及其对氯酚的脱氯作用。

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摘要

Three strains of Pseudomonas pickettii that can grow with 2,4,6-trichlorophenol (2,4,6-TCP) as the sole source of carbon and energy were isolated from different mixed cultures of soil bacterial populations that had been acclimatized to 2,4,6-TCP. These strains released 3 mol of chloride ion from 1 mol of 2,4,6-TCP during the complete degradation of the TCP. Of these strains, P. pickettii DTP0602 in high-cell-density suspension cultures dechlorinated various chlorophenols (CPs). Cells that were preincubated with 2,4,6-TCP converted isomers of 4-CP to the corresponding chloro-p-hydroquinones, but those preincubated with 4-CP converted CPs lacking a chlorine atom(s) at the o position to isomers of chlorocatechol. The ability of DTP0602 to dechlorinate 2,4,6-TCP was induced by 2,6-dichlorophenol, 2,3,6- and 2,4,6-TCP, and 2,3,4,6-tetrachlorophenol and was repressed in the presence of succinate or glucose.
机译：从已经适应环境2的土壤细菌种群的不同混合培养物中分离出三株可以以2,4,6-三氯苯酚（2,4,6-TCP）作为唯一碳和能量来源生长的Pseudomonas pickettii菌株。 4,6-TCP。在TCP完全降解期间，这些菌株从1摩尔的2,4,6-TCP释放了3摩尔的氯离子。在这些菌株中，在高细胞密度悬浮培养物中的P. pickettii DTP0602使各种氯酚（CP）脱氯。用2-CP的2,4,6-TCP转化的异构体预孵育为相应的氯-对氢醌的细胞，但是用4-CP转化的CP预先孵育的细胞在o位置缺少氯原子的异构体氯邻苯二酚。 2,6-二氯苯酚，2,3,6-和2,4,6-TCP和2,3,4,6-四氯苯酚诱导DTP0602对2,4,6-TCP脱氯的能力受到抑制在琥珀酸盐或葡萄糖存在下。

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