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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Application of antisera raised against sulfate-reducing bacteria for indirect immunofluorescent detection of immunoreactive bacteria in sediment from the German Baltic Sea.
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Application of antisera raised against sulfate-reducing bacteria for indirect immunofluorescent detection of immunoreactive bacteria in sediment from the German Baltic Sea.

机译：将针对硫酸盐还原菌的抗血清用于间接免疫荧光检测德国波罗的海沉积物中的免疫反应性细菌。

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Polyclonal rabbit antisera raised against sulfate-reducing bacteria (SRB) could detect several distinct populations of bacteria in sediment from the German Baltic Sea. The depth distribution of immunoreactive bacteria was determined by an indirect immunofluorescence filter method. Anti-Desulfovibrio desulfuricans DSM 1926 serum showed maximum bacterial numbers at a depth of 18 cm, with a concentration of 60 x 10(6) cells cm-3. With anti-Desulfovibrio baculatus DSM 2555 serum, counts were highest at the same depth, approaching 0.7 x 10(6) cells cm-3. Other significantly smaller populations were observed. Anti-SRBStrain 1 (lactate,vibrio) maxima were at 0 to 4 cm and at 17 to 18 cm. Anti-SRBStrain 2 (lactate,vibrio) serum showed several local maxima. Anti-SRBStrain 3 (lactate,oval) serum detected one single peak at a depth of 10 to 12 cm. Also determined were rates of sulfate reduction, total bacterial counts by acridine orange staining, and the viable counts by dilution series on anaerobic lactate medium. The total bacterial counts were highest (180 x 10(6) cells cm-3) at 3 to 4 cm and dropped to 24 x 10(6) cells cm-3 at 10 to 11 cm but showed additional local maxima reaching 140 x 10(6) cells cm-3 at a depth of 17 to 18 cm. Viable counts probable number) were above 10(5) CFU cm-3 at 0 to 3.6 cm but remained below 10(3) CFU at 7.2 to 18 cm. The sulfate reduction rate was maximal (107 nmol cm-3 day-1) at a depth of 1 to 2 cm, dropped to 10 nmol cm-3 day-1 at 12 to 13 cm, and reached 38 nmol cm-3 day-1 at 17 to 18 cm.
机译：针对硫酸盐还原菌（SRB）产生的多克隆兔抗血清可以检测德国波罗的海沉积物中几种不同的细菌种群。免疫反应细菌的深度分布通过间接免疫荧光滤光片法测定。抗Desulfovibrio desulfuricans DSM 1926血清在18 cm深度处显示最大细菌数，浓度为60 x 10（6）细胞cm-3。使用抗Desulfovibrio baculatus DSM 2555血清，在相同深度处的计数最高，接近0.7 x 10（6）cm-3细胞。观察到其他明显较小的种群。抗SRBStrain 1（乳酸，弧菌）最大值在0至4 cm和17至18 cm。抗SRBStrain 2（乳酸，弧菌）血清显示出几个局部最大值。抗SRBStrain 3（乳酸，卵形）血清在10至12 cm的深度检测到一个单峰。还确定了硫酸盐还原的速率，a啶橙染色的总细菌数，以及在厌氧乳酸培养基上的稀释系列的存活数。总细菌数在3至4 cm处最高（180 x 10（6）细胞cm-3），在10至11 cm处降至24 x 10（6）细胞cm-3，但显示出额外的局部最大值达到140 x 10 （6）细胞cm-3，深度为17至18 cm。可行的计数可能数）在0至3.6 cm时高于10（5）CFU cm-3，但在7.2至18 cm时仍低于10（3）CFU。硫酸盐还原速率在1至2 cm的深度处最大（107 nmol cm-3 day-1），在12至13 cm处降至10 nmol cm-3 day-1，达到38 nmol cm-3 day- 1在17至18厘米处。

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