首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Biological degradation of tannins in sericea lespedeza (Lespedeza cuneata) by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus analyzed by solid-state 13C nuclear magnetic resonance spectroscopy.
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Biological degradation of tannins in sericea lespedeza (Lespedeza cuneata) by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus analyzed by solid-state 13C nuclear magnetic resonance spectroscopy.

机译:白腐真菌Ceriporiopsis subvermispora和Cyathus stercoreus通过固态13C核磁共振波谱分析了Sericea lespedeza(Lespedeza cuneata)中单宁的生物降解。

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摘要

Leaves of sericea lespedeza exhibit a high proportion of condensed tannin, resulting in poor forage quality. The white rot fungi Ceriporiopsis subvermispora and Cyathus sterocoreus are known to preferentially degrade lignin in a variety of plants and were evaluated for their ability to degrade condensed tannin from sericea leaves with the aim of improving digestibility. Relative levels of condensed tannin, cutin, pectin, and cellulose were monitored as a function of fungal treatment by solid-state cross-polarization and magic angle spinning 13C nuclear magnetic resonance spectroscopy. Total soluble phenolics, soluble tannins, and soluble and insoluble proanthocyanidin levels in fungus-treated and control samples were measured by established chemical techniques. Results indicate that both species of fungus preferentially degrade condensed tannin and that C. subvermispora is markedly superior to C. stercoreus in this capacity.
机译:芹菜叶片中的单宁浓缩比例很高,导致草料质量差。已知白腐真菌Ceriporiopsis subvermispora和Cyathus sterocoreus会优先降解多种植物中的木质素,并对其降解能力从绢云母叶片中降解单宁进行了评估,目的是提高消化率。通过固态交叉极化和魔角旋转13C核磁共振波谱法监测了相对于真菌处理的缩合单宁,角质,果胶和纤维素的相对水平。通过建立的化学技术测量真菌处理和对照样品中的总可溶性酚类,可溶性单宁以及可溶性和不可溶性原花色素水平。结果表明,这两种真菌都优先降解缩合的单宁,而在这种能力下,深层隐孢子虫明显优于胸果隐孢子虫。

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