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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14
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Biocontrol of Rhizoctonia solani Damping-Off of Tomato with Bacillus subtilis RB14

机译：枯草芽孢杆菌RB14抑制番茄茄枯萎病的生物防治

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摘要

Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. subtilis RB14-C, a spontaneous streptomycin-resistant mutant of RB14, was used. Damping-off was suppressed when the culture broth, cell suspension, or cell-free culture broth of RB14-C was inoculated into soil. Iturin A and surfactin were recovered from the soils inoculated with the cell suspension of RB14-C, confirming that RB14-C produced them in soil. The gene lpa-14, which was cloned from RB14 and required for the production of both antibiotics, was mutated in RB14-C, and a mutant, R(Delta)1, was constructed. The level of disease suppressibility of R(Delta)1 was low, but R(Delta)1(pC115), a transformant of R(Delta)1 with the plasmid pC115 carrying lpa-14, was restored in suppressibility. These results show that the antibiotics iturin A and surfactin produced by RB14 play a major role in the suppression of damping-off caused by R. solani. RB14-C, R(Delta)1, and R(Delta)1(pC115) persisted in soil during the experimental period and were recovered from the soil, mostly as spores.
机译：枯草芽孢杆菌RB14通过生产罐头抗生素Iturin A和surfactin表现出对几种植物病原菌的体外抗菌活性，并进行了盆栽试验，以研究其抑制番茄红枯菌引起的阻尼作用。为了促进从土壤中的恢复，使用了枯草芽孢杆菌RB14-C（一种自发的抗链霉素的RB14突变体）。当将RB14-C的培养液，细胞悬液或无细胞培养液接种到土壤中时，阻尼被抑制。从接种了RB14-C细胞悬液的土壤中回收了Iturin A和surfactin，证实RB14-C在土壤中产生了它们。从RB14克隆并产生两种抗生素所需的基因lpa-14在RB14-C中突变，并构建了突变体RΔ1。 RΔ1的疾病可抑制性水平低，但是RΔ1（pC115）是具有携带lpa-14的质粒pC115的RΔ1的转化体，恢复了可抑制性。这些结果表明，由RB14产生的抗生素iturin A和surfactin在抑制由sol。R. solani引起的衰减中起主要作用。 RB14-C，RΔ1和RΔ1（pC115）在实验期间持续存在于土壤中，并从土壤中回收，大部分为孢子。

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