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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Chloroform Cometabolism by Butane-Grown CF8 Pseudomonas butanovora and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b
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Chloroform Cometabolism by Butane-Grown CF8 Pseudomonas butanovora and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b

机译：丁烷-生长的CF8丁酸假单胞菌和牛分枝杆菌JOB5和甲烷-生长的甲基肌球孢霉OB3b的氯仿代谢

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Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b. All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O(inf2). Butane inhibited CF degradation by the butane-grown bacteria, suggesting that butane monooxygenase is responsible for CF degradation. P. butanovora required exogenous reductant to degrade CF, while CF8 and M. vaccae utilized endogenous reductants. Prolonged incubation with CF resulted in decreased CF degradation. CF8 and P. butanovora were more sensitive to CF than either M. trichosporium or M. vaccae. CF degradation by all three butane-grown bacteria was inactivated by acetylene, which is a mechanism-based inhibitor for several monooxygenases. Butane protected all three butane-grown bacteria from inactivation by acetylene, which indicates that the same monooxygenase is responsible for both CF and butane oxidation. CF8 and P. butanovora were able to degrade other chlorinated hydrocarbons, including trichloroethylene, 1,2-cis-dichloroethylene, and vinyl chloride. In addition, CF8 degraded 1,1,2-trichloroethane. The results indicate the potential of butane-grown bacteria for chlorinated hydrocarbon transformation.
机译：将丁烷生长的富集培养物CF8的氯仿（CF）降解与丁烷生长的假单胞菌butanovora和牛分枝杆菌JOB5的降解以及已知CF降解剂甲基毛孢菌OB3b的降解进行了比较。所有三种丁烷生长细菌都能够以与毛孢霉菌相当的速率降解CF。所有四种细菌的CF降解都需要O（inf2）。丁烷抑制丁烷生长细菌对CF的降解，表明丁烷单加氧酶是CF降解的原因。丁酸疟原虫需要外源还原剂来降解CF，而CF8和vac。vaccae利用内源性还原剂。与CF长时间孵育会导致CF降解降低。 CF8和丁酸疟原虫对CF的敏感度高于Trichosporium或vaccae。所有三种丁烷生长细菌的CF降解都被乙炔灭活，乙炔是几种单加氧酶的一种基于机理的抑制剂。丁烷可保护所有三种丁烷生长细菌免受乙炔灭活的影响，这表明相同的单加氧酶既可引起CF氧化，又可引起丁烷氧化。 CF8和丁酸丙酸杆菌能够降解其他氯化烃，包括三氯乙烯，1，2-顺式二氯乙烯和氯乙烯。此外，CF8降解了1,1,2-三氯乙烷。结果表明丁烷生长的细菌潜在的氯代烃转化。

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