Differentiation of Campylobacter Isolates on the Basis of Sensitivity to Boiling in Water as Measured by PCR-Detectable DNA

机译：PCR检测可检测到的弯曲杆菌分离株对水中沸腾的敏感性

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Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.

机译：据报道，埃及分离出的45种空肠弯曲杆菌和弯曲杆菌菌株对在水中煮沸后释放PCR可检测的基因组DNA的敏感性不同。用蛋白酶K和十二烷基硫酸钠处理后，所有菌株均释放出PCR可检测的DNA。当通过在水中煮沸从这些菌株中提取DNA时，九个（20％）菌株PCR阴性或对煮沸具有抗性，表明存在对煮沸敏感和对煮沸具有抵抗力的表型。

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期刊名称 Applied and Environmental Microbiology
作者
Zaynab S. Mohran; Ray R. Arthur; Buhari A. Oyofo; Leonard F. Peruski; Momtaz O. Wasfy; Tharwat F. Ismail; James R. Murphy;
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年(卷),期 1998(64),1
年度 1998
页码 363–365
总页数 3
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
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1. Survival of Campylobacter jejuni Campylobacter jejuni and Campylobacter?coli Campylobacter?coli water isolates in lake and well water [J] . Nilsson Anna, Johansson Cecilia, Skarp Astrid, APMIS: Acta Pathologica, Microbiologica et Immunologica Scandinavica . 2018,第9期

机译：Campylobacter jejuni Campylobacter jejuni和Campylobacter的生存？大肠杆菌弯曲杆菌？Coli水分在湖泊和井水中
2. Differentiation of Campylobacter jejuni isolates by random amplified polymorphic DNA method. [J] . Wieczorek K, Osek J Medycyna Weterynaryjna . 2006,第6期

机译：空肠弯曲杆菌分离株的随机扩增多态性DNA方法鉴别。
3. A multiplex polymerase chain reaction to detect and differentiate Campylobacter fetus subspecies fetus and Campylobacter fetus species venerealis: use on UK isolates of C-fetus and other Campylobacter spp. [J] . Willoughby K, Nettleton PF, Quirie M, Journal of applied microbiology . 2005,第4期

机译：一种多重聚合酶链反应，可检测和区分胎儿弯曲杆菌亚种胎儿和弯曲杆菌胎儿种的性病：在英国分离的C胎儿和其他弯曲杆菌属中使用。
4. The use of mt DNA gene probes for the molecular typing and differentiation of Verticillium lecanii Isolates [C] . V. N. Kouvelis, M. A. Typas International Verticillium Symposium . 2000

机译：使用MT DNA基因探针对羊角酸盐的分子键入和分化
5. Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter. [D] . Zhang, Hai. 2006

机译：基于单核苷酸多态性的电子DNA芯片技术的发展，用于检测和分析活弯曲杆菌。
6. Antibiotic Sensitivity Profiling and Virulence Potential of Campylobacter jejuni Isolates from Estuarine Water in the Eastern Cape Province South Africa [O] . Anthony C. Otigbu, Anna M. Clarke, Justine Fri, 2018

机译：南非东开普省河口水空肠弯曲菌分离物的抗生素敏感性分析和毒力潜能
7. Differentiation and grouping of isolates of the Ganoderma lucidum complex by random amplified polymorphic DNA-PCR compared with grouping on the basis of internal transcribed spacer sequences [O] . R S Hseu, H H Wang, H F Wang, 1996

机译：随机扩增多态性DNA-PCR与基于内部转录间隔序列的分组相比，Ganoderma lucidum复合物分离株的分化和分组

Differentiation of Campylobacter Isolates on the Basis of Sensitivity to Boiling in Water as Measured by PCR-Detectable DNA

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