• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Structure and Dynamics of Experimentally Introduced and Naturally Occurring Laccaria sp. Discrete Genotypes in a Douglas Fir Plantation
【2h】

Structure and Dynamics of Experimentally Introduced and Naturally Occurring Laccaria sp. Discrete Genotypes in a Douglas Fir Plantation

机译：实验引入和自然发生的Laccaria sp。的结构和动力学。花旗松人工林的离散基因型

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ectomycorrhizal fungi have been introduced in forest nurseries to improve seedling growth. Outplanting of inoculated seedlings to forest plantations raises the questions about inoculant persistence and its effects on indigenous fungal populations. We previously showed (M.-A. Selosse et al. Mol. Ecol. 7:561–573, 1998) that the American strain Laccaria bicolor S238N persisted 10 years after outplanting in a French Douglas fir plantation, without introgression or selfing and without fruiting on uninoculated adjacent plots. In the present study, the relevance of those results to sympatric strains was assessed for another part of the plantation, planted in 1985 with seedlings inoculated with the French strain L. bicolor 81306 or left uninoculated. About 720 Laccaria sp. sporophores, collected from 1994 to 1997, were typed by using randomly amplified polymorphic DNA markers and PCR amplification of the mitochondrial and nuclear ribosomal DNAs. All plots were colonized by small spontaneous discrete genotypes (genets). The inoculant strain 81306 abundantly fruited beneath inoculated trees, with possible introgression in indigenous Laccaria populations but without selfing. In contrast to our previous survey of L. bicolor S238N, L. bicolor 81306 colonized a plot of uninoculated trees. Meiotic segregation analysis verified that the invading genet was strain 81306 (P < 0.00058), implying a vegetative growth of 1.1 m · year−1. This plot was also invaded in 1998 by strain S238N used to inoculate other trees of the plantation. Five other uninoculated plots were free of these inoculant strains. The fate of inoculant strains thus depends less on their geographic origin than on unknown local factors.
机译：外生菌根真菌已被引入森林苗圃，以改善幼苗生长。将接种后的幼苗移栽到人工林中引发了关于接种物持久性及其对土著真菌种群的影响的问题。我们之前曾证明（M.-A. Selosse等人，分子生物学杂志7：561-573，1998），美国菌株Laccaria bicolor S238N在法国道格拉斯冷杉人工林中移栽后持续了10年，没有渗水或自交，也没有在未接种的邻近地块上结果。在本研究中，对种植园的另一部分评估了这些结果与同属菌株的相关性，该种植园于1985年种植，接种了法国双色L. 81306菌株或未接种。约720 Laccaria sp。通过使用随机扩增的多态性DNA标记以及线粒体和核糖体DNA的PCR扩增，对1994年至1997年收集的孢子体进行分型。所有地块均以小型自发离散基因型（基因组）定殖。接种菌81306在接种树下结实，在土著拉卡里亚种群中可能有渗入，但没有自交。与我们先前对双色乳杆菌S238N的调查相反，双色乳杆菌81306殖民了未接种树木的地块。减数分裂分离分析证实入侵种是81306菌株（P <0.00058），表明其营养生长为1.1 m·year -1 。该地块还在1998年被S238N菌株入侵，该菌株用于接种人工林的其他树木。其他五个未接种地块均不含这些接种菌株。因此，接种菌株的命运取决于其地理起源，而不是取决于未知的本地因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号