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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Formation of Hydride-Meisenheimer Complexes of Picric Acid (246-Trinitrophenol) and 24-Dinitrophenol during Mineralization of Picric Acid by Nocardioides sp. Strain CB 22-2
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Formation of Hydride-Meisenheimer Complexes of Picric Acid (246-Trinitrophenol) and 24-Dinitrophenol during Mineralization of Picric Acid by Nocardioides sp. Strain CB 22-2

机译：诺卡氏菌矿化苦味酸中苦味酸（246-三硝基苯酚）和24-二硝基苯酚的氢化物-迈森海默配合物的形成。菌株CB 22-2

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There are only a few examples of microbial conversion of picric acid (2,4,6-trinitrophenol). None of the organisms that have been described previously is able to use this compound as a sole source of carbon, nitrogen, and energy at high rates. In this study we isolated and characterized a strain, strain CB 22-2, that was able to use picric acid as a sole source of carbon and energy at concentrations up to 40 mM and at rates of 1.6 mmol · h−1 · g (dry weight) of cells−1 in continuous cultures and 920 μmol · h−1 · g (dry weight) of cells−1 in flasks. In addition, this strain was able to use picric acid as a sole source of nitrogen at comparable rates in a nitrogen-free medium. Biochemical characterization and 16S ribosomal DNA analysis revealed that strain CB 22-2 is a Nocardioides sp. strain. High-pressure liquid chromatography and UV-visible light data, the low residual chemical oxygen demand, and the stoichiometric release of 2.9 ± 0.1 mol of nitrite per mol of picric acid provided strong evidence that complete mineralization of picric acid occurred. During transformation, the metabolites detected in the culture supernatant were the [H−]-Meisenheimer complexes of picric acid and 2,4-dinitrophenol (H−-DNP), as well as 2,4-dinitrophenol. Experiments performed with crude extracts revealed that H−-DNP formation indeed is a physiologically relevant step in picric acid metabolism.
机译：苦味酸（2,4,6-三硝基苯酚）的微生物转化只有少数例子。先前描述的任何生物都无法将这种化合物用作高速率的唯一碳，氮和能量来源。在这项研究中，我们分离并鉴定了菌株CB 22-2，该菌株能够使用苦味酸作为碳和能量的唯一来源，其浓度高达40 mM，速率为1.6 mmol·h ·连续培养的细胞 −1 的克（干重）和920μmol·h −1 ·g的细胞干 −g的干重1 在烧瓶中。此外，该菌株能够在无氮培养基中以可比的速率使用苦味酸作为唯一的氮源。生化特征和16S核糖体DNA分析表明，菌株CB 22-2是诺卡氏菌。应变。高压液相色谱法和紫外可见光数据，低的化学需氧量以及每摩尔苦味酸2.9±0.1 mol亚硝酸盐的化学计量释放提供了强有力的证据，证明发生了苦味酸完全矿化。在转化过程中，培养上清液中检测到的代谢物是苦味酸和2,4-二硝基苯酚的[H -]-Meisenheimer配合物（H - -DNP），以及2,4-二硝基苯酚用粗提取物进行的实验表明，H - -DNP的形成确实是苦味酸代谢中的生理相关步骤。

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