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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Pathway of Propionate Oxidation by a Syntrophic Culture of Smithella propionica and Methanospirillum hungatei
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Pathway of Propionate Oxidation by a Syntrophic Culture of Smithella propionica and Methanospirillum hungatei

机译：丙酸史密氏菌和饥饿感甲基螺旋藻同养培养的丙酸氧化途径

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摘要

The pathway of propionate conversion in a syntrophic coculture of Smithella propionica and Methanospirillum hungatei JF1 was investigated by 13C-NMR spectroscopy. Cocultures produced acetate and butyrate from propionate. [3-13C]propionate was converted to [2-13C]acetate, with no [1-13C]acetate formed. Butyrate from [3-13C]propionate was labeled at the C2 and C4 positions in a ratio of about 1:1.5. Double-labeled propionate (2,3-13C) yielded not only double-labeled acetate but also single-labeled acetate at the C1 or C2 position. Most butyrate formed from [2,3-13C]propionate was also double labeled in either the C1 and C2 atoms or the C3 and C4 atoms in a ratio of about 1:1.5. Smaller amounts of single-labeled butyrate and other combinations were also produced. 1-13C-labeled propionate yielded both [1-13C]acetate and [2-13C]acetate. When 13C-labeled bicarbonate was present, label was not incorporated into acetate, propionate, or butyrate. In each of the incubations described above, 13C was never recovered in bicarbonate or methane. These results indicate that S. propionica does not degrade propionate via the methyl-malonyl-coenzyme A (CoA) pathway or any other of the known pathways, such as the acryloyl-CoA pathway or the reductive carboxylation pathway. Our results strongly suggest that propionate is dismutated to acetate and butyrate via a six-carbon intermediate.
机译：用 13 C-NMR光谱法研究了丙酸史密氏菌和饥饿的甲基甲烷螺旋菌JF1的共养过程中丙酸转化途径。共培养从丙酸酯产生乙酸盐和丁酸盐。 [3- 13 C]丙酸酯被转化为[2- 13 C]乙酸酯，没有形成[1- 13 C]乙酸酯。来自[3- 13 C]丙酸酯的丁酸酯在C2和C4位置的比例约为1：1.5。双标记的丙酸酯（2,3- 13 C）不仅在C1或C2位置生成双标记的乙酸盐，而且生成单标记的乙酸盐。由[2,3- 13 C]丙酸酯形成的大多数丁酸酯也被双标记在C1和C2原子或C3和C4原子中，比例约为1：1.5。还产生了少量的单标记丁酸酯和其他组合。 1- 13 C标记的丙酸酯生成[1- 13 C]乙酸盐和[2- 13 C]乙酸盐。当存在 13 C标记的碳酸氢盐时，标记未掺入乙酸盐，丙酸盐或丁酸盐中。在上述每次温育中，从未在碳酸氢盐或甲烷中回收 13 C。这些结果表明丙酸链球菌不会通过甲基-丙二酰辅酶A（CoA）途径或任何其他已知途径（如丙烯酰基-CoA途径或还原性羧化途径）降解丙酸酯。我们的研究结果强烈表明，丙酸酯通过六碳中间体转变为乙酸酯和丁酸酯。

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