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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Numerical Dominance and Phylotype Diversity of Marine Rhodobacter Species during Early Colonization of Submerged Surfaces in Coastal Marine Waters as Determined by 16S Ribosomal DNA Sequence Analysis and Fluorescence In Situ Hybridization
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Numerical Dominance and Phylotype Diversity of Marine Rhodobacter Species during Early Colonization of Submerged Surfaces in Coastal Marine Waters as Determined by 16S Ribosomal DNA Sequence Analysis and Fluorescence In Situ Hybridization

机译：通过16S核糖体DNA序列分析和荧光原位杂交确定沿海红海水下表面早期定殖期间海洋红细菌物种的数量优势和型型多样性

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Early stages of surface colonization in coastal marine waters appear to be dominated by the marine Rhodobacter group of the α subdivision of the division Proteobacteria (α-Proteobacteria). However, the quantitative contribution of this group to primary surface colonization has not been determined. In this study, glass microscope slides were incubated in a salt marsh tidal creek for 3 or 6 days. Colonizing bacteria on the slides were examined by fluorescence in situ hybridization by employing DNA probes targeting 16S or 23S rRNA to identify specific phylogenetic groups. Confocal laser scanning microscopy was then used to quantify and track the dynamics of bacterial primary colonists during the early stages of surface colonization and growth. More than 60% of the surface-colonizing bacteria detectable by fluorescence staining (Yo-Pro-1) could also be detected with the Bacteria domain probe EUB338. Archaea were not detected on the surfaces and did not appear to participate in surface colonization. Of the three subdivisions of the Proteobacteria examined, the α-Proteobacteria were the most abundant surface-colonizing organisms. More than 28% of the total bacterial cells and more than 40% of the cells detected by EUB338 on the surfaces were affiliated with the marine Rhodobacter group. Bacterial abundance increased significantly on the surfaces during short-term incubation, mainly due to the growth of the marine Rhodobacter group organisms. These results demonstrated the quantitative importance of the marine Rhodobacter group in colonization of surfaces in salt marsh waters and confirmed that at least during the early stages of colonization, this group dominated the surface-colonizing bacterial assemblage.
机译：在沿海海水中，表面定殖的早期阶段似乎由变形杆菌科（α-Proteobacteria）的α细分的海洋红细菌群控制。但是，该组对主要表面定殖的定量贡献尚未确定。在这项研究中，将玻璃显微镜载玻片在盐沼潮汐河中孵育3或6天。通过使用靶向16S或23S rRNA的DNA探针鉴定特定的系统发生基团，通过荧光原位杂交技术检查载玻片上的细菌。然后使用共聚焦激光扫描显微镜对表面定殖和生长的早期阶段细菌初级定殖者的动力学进行定量和跟踪。还可通过细菌域探针EUB338检测到超过60％的可通过荧光染色（Yo-Pro-1）检测到的表面克隆细菌。在表面上未检测到古细菌，并且似乎未参与表面定植。在所检测的变形杆菌的三个细分中，α-变形杆菌是最丰富的表面定殖生物。海洋细菌性红细菌菌群与总细菌细胞中的28％以上以及EUB338在表面上检测到的细胞中的40％以上有关。短期孵育期间，细菌表面的细菌丰度显着增加，这主要是由于海洋红细菌群生物的生长所致。这些结果证明了海洋红细菌菌群在盐沼水域表面定殖中的定量重要性，并证实至少在定殖的早期阶段，该组支配了表面定殖的细菌组合。

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