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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Colonization of Vitis vinifera by a Green Fluorescence Protein-Labeled gfp-Marked Strain of Xylophilus ampelinus the Causal Agent of Bacterial Necrosis of Grapevine
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Colonization of Vitis vinifera by a Green Fluorescence Protein-Labeled gfp-Marked Strain of Xylophilus ampelinus the Causal Agent of Bacterial Necrosis of Grapevine

机译：通过绿色荧光蛋白标记的gfp标记的木糖Xylophilus ampelinus菌株对葡萄葡萄的定殖Apelinus是葡萄细菌性坏死的病原体

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The dynamics of Xylophilus ampelinus were studied in Vitis vinifera cv. Ugni blanc using gfp-marked bacterial strains to evaluate the relative importance of epiphytic and endophytic phases of plant colonization in disease development. Currently, bacterial necrosis of grapevine is of economic importance in vineyards in three regions in France: the Cognac, Armagnac, and Die areas. This disease is responsible for progressive destruction of vine shoots, leading to their death. We constructed gfp-marked strains of the CFBP2098 strain of X. ampelinus for histological studies. We studied the colonization of young plants of V. vinifera cv. Ugni blanc by X. ampelinus after three types of artificial contamination in a growth chamber and in a greenhouse. (i) After wounding of the stem and inoculation, the bacteria progressed down to the crown through the xylem vessels, where they organized into biofilms. (ii) When the bacteria were forced into woody cuttings, they rarely colonized the emerging plantlets. Xylem vessels could play a key role in the multiplication and conservation of the bacteria, rather than being a route for plant colonization. (iii) When bacterial suspensions were sprayed onto the plants, bacteria progressed in two directions: both in emerging organs and down to the crown, thus displaying the importance of epiphytic colonization in disease development.
机译：在Vitis vinifera cv中研究了Xylophilus ampelinus的动力学。 Ugni blanc使用gfp标记的细菌菌株评估植物定植的附生阶段和内生阶段在疾病发展中的相对重要性。目前，在法国三个地区的葡萄园中，葡萄的细菌坏死具有重要的经济意义：科涅克白兰地，阿尔马尼亚克和迪埃地区。这种疾病导致藤蔓芽的逐渐破坏，导致其死亡。我们构建了X. ampelinus CFBP2098菌株的gfp标记菌株，用于组织学研究。我们研究了V. vinifera cv的年轻植物的定殖。 X. ampelinus的Ugni blanc在生长室和温室中进行了三种人工污染后。 （i）茎受伤并接种后，细菌通过木质部血管向下扩散到冠部，并在那里组织成生物膜。 （ii）当细菌被迫进入木屑时，它们很少在新兴的小植株上定殖。木质部容器可以在细菌的繁殖和保存中发挥关键作用，而不是成为植物定殖的途径。 （iii）当将细菌悬浮液喷洒到植物上时，细菌向两个方向发展：在新兴器官中，一直向下到树冠，因此显示了附生菌落在疾病发展中的重要性。

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