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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Prevalence and Characterization of Shiga Toxin-Producing Escherichia coli in Swine Feces Recovered in the National Animal Health Monitoring Systems Swine 2000 Study
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Prevalence and Characterization of Shiga Toxin-Producing Escherichia coli in Swine Feces Recovered in the National Animal Health Monitoring Systems Swine 2000 Study

机译：在国家动物健康监测系统的猪2000研究中回收的产生于志贺毒素的大肠杆菌在猪粪中的流行和特征

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A study was conducted to determine the prevalence of Shiga toxin-producing Escherichia coli (STEC) in swine feces in the United States as part of the National Animal Health Monitoring System's Swine 2000 study. Fecal samples collected from swine operations from 13 of the top 17 swine-producing states were tested for the presence of STEC. After enrichment of swine fecal samples in tryptic soy broth, the samples were tested for the presence of stx1 and stx2 by use of the TaqMan E. coli STX1 and STX2 PCR assays. Enrichments of samples positive for stx1 and/or stx2 were plated, and colony hybridization was performed using digoxigenin-labeled probes complementary to the stx1 and stx2 genes. Positive colonies were picked and confirmed by PCR for the presence of the stx1, stx2, or stx2e genes, and the isolates were serotyped. Out of 687 fecal samples tested using the TaqMan assays, 70% (484 of 687) were positive for Shiga toxin genes, and 54% (370 of 687), 64% (436 of 687), and 38% (261 of 687) were positive for stx1, stx2, and both toxin genes, respectively. Out of 219 isolates that were characterized, 29 (13%) produced stx1, 14 (6%) produced stx2, and 176 (80%) produced stx2e. Twenty-three fecal samples contained at least two STEC strains that had different serotypes but that had the same toxin genes or included a strain that possessed stx1 in addition to a strain that possessed stx2 or stx2e. The STEC isolates belonged to various serogroups, including O2, O5, O7, O8, O9, OX10, O11, O15, OX18, O20, O57, O65, O68, O69, O78, O91, O96, O100, O101, O120, O121, O152, O159, O160, O163, and O untypeable. It is noteworthy that no isolates of serogroup O157 were recovered. Results of this study indicate that swine in the United States harbor STEC that can potentially cause human illness.
机译：作为国家动物健康监测系统“猪2000”研究的一部分，进行了一项研究以确定在美国猪粪中产生志贺毒素的大肠杆菌（STEC）的患病率。测试了从前17个产猪州中的13个州的猪场收集的粪便样品中是否存在STEC。在胰蛋白酶大豆肉汤中富集猪粪便样品后，使用TaqMan大肠杆菌STX1和STX2 PCR分析法检测样品中stx1和stx2的存在。平板接种stx1和/或stx2阳性的样品，并用洋地黄毒苷标记的与stx1和stx2基因互补的探针进行菌落杂交。挑出阳性菌落，并通过PCR确认stx1，stx2或stx2e基因的存在，并对分离株进行血清分型。使用TaqMan分析测试的687份粪便样本中，志贺毒素基因呈阳性的占70％（687的484），分别为54％（687的370），64％（687的436）和38％（687的261）。分别对stx1，stx2和两个毒素基因呈阳性。在鉴定出的219种分离株中，有29（13％）个产生stx1，14（6％）产生stx2，176个（80％）产生stx2e。 23个粪便样本中至少包含两个STEC菌株，这些菌株具有不同的血清型，但具有相同的毒素基因，或者除具有 stx 的菌株外还具有 stx 1的菌株。 em> 2或 stx 2e 。 STEC分离物属于各种血清群，包括O2，O5，O7，O8，O9，OX10，O11，O15，OX18，O20，O57，O65，O68，O69，O78，O91，O96，O100，O101，O120，O121 ，O152，O159，O160，O163和O无法键入。值得注意的是，没有回收到O157血清群。这项研究的结果表明，美国的猪具有可能引起人类疾病的STEC。

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