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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >High-Level Genotypic Variation and Antibiotic Sensitivity among Escherichia coli O157 Strains Isolated from Two Scottish Beef Cattle Farms
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High-Level Genotypic Variation and Antibiotic Sensitivity among Escherichia coli O157 Strains Isolated from Two Scottish Beef Cattle Farms

机译：从两个苏格兰肉牛场分离的大肠杆菌O157菌株之间的高水平基因型变异和抗生素敏感性

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Escherichia coli O157:H7 is a human pathogen that is carried and transmitted by cattle. Scotland is known to have one of the highest rates of E. coli O157 human infections in the world. Two hundred ninety-three isolates were obtained from naturally infected cattle and the environment on two farms in the Scottish Highlands. The isolates were typed by pulsed-field gel electrophoresis (PFGE) with XbaI restriction endonuclease enzyme, and 19 different variations in patterns were found. There was considerable genomic diversity within the E. coli O157 population on the two farms. The PFGE pattern of one of the observed subtypes matched exactly with that of a strain obtained from a Scottish patient with hemolytic-uremic syndrome. To examine the stability of an individual E. coli O157 strain, continuous subculturing of a strain was performed 110 times. No variation from the original PFGE pattern was observed. We found three indistinguishable subtypes of E. coli O157 on both study farms, suggesting common sources of infection. We also examined the antibiotic resistance of the isolated strains. Phenotypic studies demonstrated resistance of the strains to sulfamethoxazole (100%), chloramphenicol (3.07%), and at a lower rate, other antibiotics, indicating the preservation of antibiotic sensitivity in a rapidly changing population of E. coli O157.
机译：大肠杆菌O157：H7是由牛携带和传播的人类病原体。众所周知，苏格兰是全球O157人大肠杆菌感染率最高的国家之一。在苏格兰高地的两个农场中，从自然感染的牛和环境中获得了293株分离株。通过XbaI限制性核酸内切酶的脉冲场凝胶电泳（PFGE）对分离物进行分型，发现19种不同的模式变异。在这两个农场的O157大肠杆菌种群中存在相当大的基因组多样性。观察到的亚型之一的PFGE模式与从患有溶血性尿毒症综合征的苏格兰患者获得的菌株的PFGE模式完全匹配。为了检查单个大肠杆菌O157菌株的稳定性，将菌株连续传代培养110次。没有观察到与原始PFGE模式的差异。我们在两个研究农场中都发现了三种不可区分的大肠杆菌O157亚型，表明常见的感染源。我们还检查了分离菌株的抗生素抗性。表型研究表明，该菌株对磺胺甲恶唑（100％），氯霉素（3.07％）和其他抗生素的耐药率较低，表明在快速变化的大肠杆菌O157种群中，抗生素敏感性得以保持。

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