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Application of a Newly Developed ARB Software-Integrated Tool for In Silico Terminal Restriction Fragment Length Polymorphism Analysis Reveals the Dominance of a Novel pmoA Cluster in a Forest Soil

机译:用于计算机终端限制性片段长度多态性分析的最新开发的ARB软件集成工具的应用揭示了森林土壤中新型pmoA簇的优势

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摘要

TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool to perform directed terminal restriction fragment length polymorphism analysis of pmoA products obtained from a forest soil revealed that novel cluster I methanotrophic bacteria were dominant.
机译:开发了TRF-CUT(一种ARB实现的工具),可在计算机上预测比对的小亚基rRNA基因或功能基因序列的末端限制性片段。应用这种新工具对从森林土壤中获得的pmoA产物进行定向的末端限制性片段长度多态性分析,发现新的簇I甲基营养型细菌占主导地位。

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