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Cloning and Expression of Two Crystal Protein Genes cry30Ba1 and cry44Aa1 Obtained from a Highly Mosquitocidal Strain Bacillus thuringiensis subsp. entomocidus INA288

机译：从高度灭蚊菌株苏云金芽孢杆菌亚种获得的两个晶体蛋白基因cry30Ba1和cry44Aa1的克隆和表达。昆虫科INA288

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摘要

Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

机译：从苏云金芽孢杆菌亚种克隆了两个新的晶体蛋白基因cry30Ba和cry44Aa。肠线虫INA288，并在无结晶菌株中表达。 Cry44Aa晶体对二龄库蚊（50％死亡率[LC50] = 6 ng / ml）和埃及伊蚊（LC50 = 12 ng / ml）有剧毒。但是，Cry30Ba晶体无毒。

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期刊名称 Applied and Environmental Microbiology
作者
Takeshi Ito; Tomonori Ikeya; Ken Sahara; Hisanori Bando; Shin-ichiro Asano;
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作者单位

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年(卷),期 2006(72),8
年度 2006
页码 5673–5676
总页数 4
原文格式 PDF
正文语种
中图分类应用微生物学;微生物学;
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专利

1. Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1, Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288 [J] . Takeshi Ito, Tomonori Ikeya, Ken Sahara, Applied and Environmental Microbiology . 2006,第8期

机译：从高度灭蚊菌株苏云金芽孢杆菌亚种获得的两个晶体蛋白基因cry30Ba1和cry44Aa1的克隆和表达。昆虫科INA288
2. Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus. [J] . B S Shin, S H Park, S K Choi, Applied and Environmental Microbiology . 1995,第6期

机译：苏云金芽孢杆菌cryV型杀虫蛋白基因的分布及苏云金芽孢杆菌亚种cryV型基因的克隆。库尔斯塔基和苏云金芽孢杆菌亚种。昆虫。
3. Cloning and expression of novel crystal protein genes cry39A and 39orf2 from Bacillus thuringiensis subsp. aizawai Bun1-14 encoding mosquitocidal proteins [J] . Takeshi Ito, Ken Sahara, Hisanori Bando, Journal of insect biotechnology and sericology . 2002,第3期

机译：苏云金芽孢杆菌亚种新型晶体蛋白基因cry39A和39orf2的克隆与表达。 aizawai Bun1-14编码灭蚊蛋白
4. Process of Action of Dipteran-Specific Insecticidal Crystal Proteins from Bacillus thuringiensis subsp. israelensis [C] . Hiroshi Sakai, Masashi Yamagiwa Pan-Pacific Conference on Pesticide Science . 2005

机译：甲硝酸芽孢杆菌患者的Diperan特异性杀虫晶体蛋白的作用方法。以色列人
5. Bacillus thuringiensis strain M15, a novel autoagglutinable, nonserotypeable strain-cloning and characterization of a novel cry31A-type crystal protein gene, cry31Aa2, and two new insertion sequences, IS231M and -N. [D] . Jung, Yong-Chul. 2002

机译：苏云金芽孢杆菌菌株M15，一种新型的可自动凝集，不可血清分型的菌株克隆，并鉴定了一种新型cry31A型晶体蛋白基因cry31Aa2以及两个新的插入序列IS231M和-N。
6. Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus. [O] . B S Shin, S H Park, S K Choi, 1995

机译：苏云金芽孢杆菌cryV型杀虫蛋白基因的分布及苏云金芽孢杆菌亚种cryV型基因的克隆。库尔斯塔基和苏云金芽孢杆菌亚种。昆虫。
7. Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1, Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288 [O] . Ito, Takeshi, Ikeya, Tomonori, Sahara, Ken, 2006

机译：从高度灭蚊菌株苏云金芽孢杆菌亚种获得的两个晶体蛋白基因cry30Ba1和cry44Aa1的克隆和表达。昆虫科INA288
8. Vertebrate Toxicology of the Solubilized Parasporal Crystalline Proteins of 'Bacillus thuringiensis' Subsp. 'israelensis' [R] . Roe, R. M., Kallapur, V. L., Dauterman, W. C., 1992

机译：“苏云金芽孢杆菌”亚种溶解的伴随结晶蛋白的脊椎动物毒理学研究。 “的以色列”

Cloning and Expression of Two Crystal Protein Genes cry30Ba1 and cry44Aa1 Obtained from a Highly Mosquitocidal Strain Bacillus thuringiensis subsp. entomocidus INA288

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