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Bacterial Community in the Crop of the Hoatzin a Neotropical Folivorous Flying Bird

机译:新热带叶飞鸟Hoatzin作物中的细菌群落

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摘要

The hoatzin is unique among known avian species because of the fermentative function of its enlarged crop. A small-bodied flying foregut fermenter is a paradox, and this bird provides an interesting model to examine how diet selection and the gut microbiota contribute to maximizing digestive efficiency. Therefore, we characterized the bacterial population in the crop of six adult hoatzins captured from the wild. A total of 1,235 16S rRNA gene sequences were grouped into 580 phylotypes (67% of the pooled species richness sampled, based on Good's coverage estimator, with CACE and Chao1 estimates of 1,709 and 1,795 species-level [99% identity] operational taxonomic units, respectively). Members of 9 of the ∼75 known phyla in Bacteria were identified in this gut habitat; the Firmicutes were dominant (67% of sequences, belonging to the classes Clostridia, Mollicutes, and Bacilli), followed by the Bacteroidetes (30%, mostly in the order Bacteroidales), Proteobacteria (1.8%), and Lentisphaerae, Verrucomicrobia, TM7, Spirochaetes, Actinobacteria, and Aminanaerobia (all <0.1%). The novelty in this ecosystem is great; 94% of the phylotypes were unclassified at the “species” level and thus likely include novel cellulolytic lineages.
机译:由于其扩大的农作物具有发酵功能,因此在已知的鸟类中,hoatzin是独特的。一个小型的飞行前肠发酵罐是一个悖论,这只鸟提供了一个有趣的模型来检查饮食选择和肠道菌群如何有助于最大程度地提高消化效率。因此,我们表征了从野外捕获的六种成年厚朴作物中的细菌种群。总共1,235个16S rRNA基因序列被分组为580个系统型(根据Good's coverage估算器,在所采样的库物种丰富度中占67%),CACE和Chao1估计为1,709个和1,795个物种水平(99%相同)的操作分类单位,分别)。在该肠道栖息地中鉴定出细菌中约75个已知门中的9个。硬毛虫占主导地位(67%的序列,属于梭菌属,莫利卡特斯和芽孢杆菌类),其次是拟杆菌属(占30%,主要是杆菌科),变形杆菌(1.8%)和扁豆菌,Verrucomicrobia,TM7,螺旋藻,放线菌和氨基厌氧菌(均<0.1%)。这个生态系统的新颖性很棒; 94%的系统型在“物种”水平上未被分类,因此可能包括新的纤维素分解谱系。

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