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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups
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Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups

机译：基于生物处理对主要粪便微生物群影响的猪粪污染标记选择

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The objective of this study was to identify a microbial marker for pig manure contamination. We quantified the persistence of four dominant bacterial groups from the pig intestinal tract throughout manure handling at 10 livestock operations (including aerobic digestion) by using molecular typing. The partial 16S rRNA genes of Bacteroides-Prevotella, Eubacterium-Clostridiaceae, Bacillus-Streptococcus-Lactobacillus (BSL), and Bifidobacterium group isolates were amplified and analyzed by capillary electrophoresis single-strand conformation polymorphism. The most dominant bacterial populations were identified by cloning and sequencing their 16S rRNA genes. The results showed that Bifidobacterium spp. and, to a lesser extent, members of the BSL group, were less affected by the aerobic treatment than either Eubacterium-Clostridiaceae or Bacteroides-Prevotella. Two Bifidobacterium species found in raw manure were still present in manure during land application, suggesting that they can survive outside the pig intestinal tract and also survive aerobic treatment. The 16S-23S rRNA internal transcribed spacer of one species, Bifidobacterium thermacidophilum subsp. porcinum, was sequenced, and a specific pair of primers was designed for its detection in the environment. With this nested PCR assay, this potential marker was not detected in samples from 30 bovine, 30 poultry, and 28 human fecal samples or in 15 urban wastewater effluents. As it was detected in runoff waters after spreading of pig manure, we propose this marker as a suitable microbial indicator of pig manure contamination.
机译：这项研究的目的是确定猪粪污染的微生物标志物。通过使用分子分型，我们对10个家畜手术（包括有氧消化）中粪肥处理过程中猪肠道中四个优势细菌群的持久性进行了量化。通过毛细管电泳单链构象多态性扩增并分析了拟杆菌，短杆菌，真细菌，大肠菌科，芽孢杆菌-链球菌-乳杆菌（BSL）和双歧杆菌组的部分16S rRNA基因。通过克隆和测序其16S rRNA基因，鉴定出最主要的细菌种群。结果表明双歧杆菌属。而且，有氧处理对BSL组成员的影响较小，而比真细菌-梭状芽孢杆菌科或拟杆菌-普列沃特杆菌少。在土地施用过程中，粪肥中仍存在两种在原始粪肥中发现的双歧杆菌物种，这表明它们可以在猪肠道外存活，也可以在需氧处理后存活。一种物种双歧杆菌嗜热嗜酸菌亚种的16S-23S rRNA内部转录间隔子。对马铃薯进行了测序，并设计了一对特定的引物用于在环境中进行检测。使用此嵌套式PCR分析法，在30头牛，30头家禽和28个人粪便样本或15种城市废水中未检测到该潜在标记。由于在猪粪传播后在径流水中检测到它，我们建议将此标记物作为猪粪污染的微生物指示剂。

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