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Spatiotemporal Variations in Microcystin Concentrations and in the Proportions of Microcystin-Producing Cells in Several Microcystis aeruginosa Populations

机译:铜绿微囊藻种群中微囊藻毒素浓度和产生微囊藻毒素的细胞比例的时空变化

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摘要

With the aim of explaining the variations in microcystin (MC) concentrations during cyanobacterial blooms, we studied several Microcystis aeruginosa populations blooming in different freshwater ecosystems located in the same geographical area. As assessed by real-time PCR, it appeared that the potentially MC-producing cells (mcyB+) were predominant (70 to 100%) in all of these M. aeruginosa populations, with the exception of one population in which non-MC-producing cells always dominated. Apart from the population in the Grangent Reservoir, we found that the proportions of potentially MC-producing and non-MC-producing cells varied little over time, which was consistent with the fact that according to a previous study of the same populations, the intergenic transcribed spacer (ITS) genotype composition did not change (). In the Grangent Reservoir, the MC-RR variant was the dominant microcystin variant throughout the bloom season, despite changes in the ITS composition and in the proportions of mcyB+ cells. Finally, the variations in total MC concentrations (0.3 to 15 μg liter−1) and in the MC cellular quotas (0.01 to 3.4 pg cell−1) were high both between and within sites, and no correlation was found between the MC concentrations and the proportion of mcyB+ cells. All of these findings demonstrate that very different results can be found for the proportions of potentially MC-producing and non-MC-producing cells and MC concentrations, even in M. aeruginosa populations living in more or less connected ecosystems, demonstrating the importance of the effect of very local environmental conditions on these parameters and also the difficulty of predicting the potential toxicity of Microcystis blooms.
机译:为了解释蓝藻水华期间微囊藻毒素(MC)浓度的变化,我们研究了位于同一地理区域的不同淡水生态系统中的若干铜绿微囊藻种群。通过实时PCR评估,似乎在所有这些铜绿假单胞菌种群中,潜在产生MC的细胞(mcyB + )占主导地位(70%至100%)非生产MC的细胞始终占主导的种群。除了格兰金水库中的种群外,我们发现潜在产生MC和非产生MC的细胞的比例随时间变化不大,这与以下事实一致:根据先前对相同种群的研究,转录间隔区(ITS)基因型组成没有变化()。在Grangent水库中,尽管ITS组成和mcyB + 细胞的比例发生了变化,但MC-RR变体是整个开花季节的主要微囊藻毒素变体。最后,两者之间的总MC浓度(0.3至15μg升 -1 )和MC细胞配额(0.01至3.4 pg cell -1 )之间的变化都很大。并且在位点内,MC浓度与mcyB + 细胞比例之间没有相关性。所有这些发现表明,即使在生活在或多或少相互联系的生态系统中的铜绿假单胞菌种群中,可能产生MC和不产生MC的细胞的比例和MC浓度也可以找到截然不同的结果,这表明局部环境条件对这些参数的影响,以及难以预测微囊藻开花的潜在毒性。

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