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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Novel NiFe- and FeFe-Hydrogenase Gene Transcripts Indicative of Active Facultative Aerobes and Obligate Anaerobes in Earthworm Gut Contents
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Novel NiFe- and FeFe-Hydrogenase Gene Transcripts Indicative of Active Facultative Aerobes and Obligate Anaerobes in Earthworm Gut Contents

机译：新型NiFe-和FeFe-加氢酶基因转录物表明worm肠道内容物中活跃的兼性需氧菌和专性厌氧菌

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摘要

The concomitant occurrence of molecular hydrogen (H2) and organic acids along the alimentary canal of the earthworm is indicative of ongoing fermentation during gut passage. Fermentative H2 production is catalyzed by [FeFe]-hydrogenases and group 4 [NiFe]-hydrogenases in obligate anaerobes (e.g., Clostridiales) and facultative aerobes (e.g., Enterobacteriaceae), respectively, functional groups that might respond differently to contrasting redox conditions. Thus, the objectives of this study were to assess the redox potentials of the alimentary canal of Lumbricus terrestris and analyze the hydrogenase transcript diversities of H2 producers in glucose-supplemented gut content microcosms. Although redox potentials in the core of the alimentary canal were variable on an individual worm basis, average redox potentials were similar. The lowest redox potentials occurred in the foregut and midgut regions, averaging 40 and 110 mV, respectively. Correlation plots between hydrogenase amino acid sequences and 16S rRNA gene sequences indicated that closely related hydrogenases belonged to closely related taxa, whereas distantly related hydrogenases did not necessarily belong to distantly related taxa. Of 178 [FeFe]-hydrogenase gene transcripts, 177 clustered in 12 Clostridiales-affiliated operational taxonomic units, the majority of which were indicative of heretofore unknown hydrogenases. Of 86 group 4 [NiFe]-hydrogenase gene transcripts, 79% and 21% were affiliated with organisms in the Enterobacteriaceae and Aeromonadaceae, respectively. The collective results (i) suggest that fermenters must cope with variable and moderately oxidative redox conditions along the alimentary canal, (ii) demonstrate that heretofore undetected hydrogenases are present in the earthworm gut, and (iii) corroborate previous findings implicating Clostridiaceae and Enterobacteriaceae as active fermentative taxa in earthworm gut content.
机译：along的消化道中同时出现分子氢（H2）和有机酸，表明肠道通过过程中正在进行发酵。在专性厌氧菌（如梭状芽孢杆菌）和兼性需氧菌（如肠杆菌科）中，[FeFe]-加氢酶和第4组[NiFe]-加氢酶分别催化发酵产生的H2，这些官能团可能对不同的氧化还原条件有不同的反应。因此，本研究的目的是评估ter虫消化道的氧化还原电位，并分析在葡萄糖补充的肠道含量微观世界中H2生产者的氢化酶转录本多样性。尽管消化道核心的氧化还原电位因蠕虫而异，但平均氧化还原电位却相似。氧化还原电位最低的发生在前肠和中肠区域，平均分别为40和110 mV。氢化酶氨基酸序列与16S rRNA基因序列之间的相关图表明，密切相关的氢化酶属于密切相关的分类单元，而远距离相关的氢化酶并不一定属于远近分类单元。在178个[FeFe]加氢酶基因转录本中，有177个聚集在12个与梭菌相关的操作分类单位中，其中大多数指示迄今未知的加氢酶。在86个第4组[NiFe]-氢化酶基因转录本中，分别有79％和21％与肠杆菌科和气单胞菌科的生物有关。总体结果（i）提示发酵罐必须应对消化道沿线的可变氧化和中等氧化还原条件，（ii）证明迄今为止在g肠中未发现过氢酶，并且（iii）证实了与梭菌和肠杆菌科有关的先前发现。 worm肠道中活跃的发酵类群。

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