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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Chemotaxis of Marinobacter adhaerens and Its Impact on Attachment to the Diatom Thalassiosira weissflogii
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Chemotaxis of Marinobacter adhaerens and Its Impact on Attachment to the Diatom Thalassiosira weissflogii

机译：马氏杆菌的趋化性及其对硅藻Thalasiosira weissflogii附着的影响

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摘要

Alga-bacterium interactions are crucial for aggregate formation and carbon cycling in aquatic systems. To understand the initiation of these interactions, we investigated bacterial chemotaxis within a bilateral model system. Marinobacter adhaerens HP15 has been demonstrated to attach to the diatom Thalassiosira weissflogii and induce transparent exopolymeric particle and aggregate formation. M. adhaerens possesses one polar flagellum and is highly motile. Bacterial cells were attracted to diatom cells, as demonstrated by addition of diatom cell homogenate or diatom culture supernatant to soft agar, suggesting that chemotaxis might be important for the interaction of M. adhaerens with diatoms. Three distinct chemotaxis-associated gene clusters were identified in the genome sequence of M. adhaerens, with the clusters showing significant sequence similarities to those of Pseudomonas aeruginosa PAO1. Mutations in the genes cheA, cheB, chpA, and chpB, which encode histidine kinases and methylesterases and which are putatively involved in either flagellum-associated chemotaxis or pilus-mediated twitching motility, were generated and mutants with the mutations were phenotypically analyzed. ΔcheA and ΔcheB mutants were found to be swimming deficient, and all four mutants were impaired in biofilm formation on abiotic surfaces. Comparison of the HP15 wild type and its chemotaxis mutants in cocultures with the diatom revealed that the fraction of bacteria attaching to the diatom decreased significantly for mutants in comparison to that for the wild type. Our results highlight the importance of M. adhaerens chemotaxis in initiation of its interaction with the diatom. In-depth knowledge of these basic processes in interspecies interactions is pivotal to obtain a systematic understanding of organic matter flux and nutrient cycling in marine ecosystems.
机译：藻类细菌的相互作用对于水生系统中聚集体的形成和碳循环至关重要。为了理解这些相互作用的开始，我们研究了双边模型系统内的细菌趋化性。业已证明，Marinobacter adhaerens HP15可附着在硅藻Thalassiosira weissflogii上并诱导透明的外聚合颗粒和聚集体形成。 adhaerens分枝杆菌拥有一种极鞭毛，并且运动能力强。细菌细胞被硅藻细胞吸引，这是通过将硅藻细胞匀浆或硅藻培养物上清液添加到软琼脂中来证明的，这表明趋化性可能对沙生分枝杆菌与硅藻的相互作用很重要。在adhaerens的基因组序列中鉴定出三个不同的趋化性相关基因簇，该簇显示出与铜绿假单胞菌PAO1的序列相似性。 cheA，cheB，chpA和chpB基因中的突变，它们编码组氨酸激酶和甲基酯酶，并可能与鞭毛相关的趋化性或菌毛介导的抽搐性有关，并产生了具有突变的表型。发现ΔcheA和ΔcheB突变体游动不足，并且所有四个突变体在非生物表面的生物膜形成中均受到损害。与硅藻共培养的HP15野生型及其趋化性突变体的比较显示，与野生型相比，突变体上附着在硅藻上的细菌比例显着降低。我们的研究结果突出了adhaerens趋化性在与硅藻相互作用中的重要性。深入了解物种间相互作用中的这些基本过程，对于系统地了解海洋生态系统中的有机物通量和养分循环至关重要。

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