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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Genomic and Physiological Characterization of the Verrucomicrobia Isolate Diplosphaera colitermitum gen. nov. sp. nov. Reveals Microaerophily and Nitrogen Fixation Genes
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Genomic and Physiological Characterization of the Verrucomicrobia Isolate Diplosphaera colitermitum gen. nov. sp. nov. Reveals Microaerophily and Nitrogen Fixation Genes

机译：Verrucomicrobia分离Diplosphaera colitermitum gen的基因组和生理学表征。十一月sp。十一月揭示了微需氧和固氮基因

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摘要

Previously we reported the cultivation of novel verrucomicrobia, including strain TAV2 (93% 16S rRNA gene identity to its nearest cultivated representative, Opitutus terreae PB90-1) from the gut of the termite Reticulitermes flavipes. To gain better insight into the Verrucomicrobia as a whole and understand the role of verrucomicrobia within the termite gut ecosystem, we analyzed a draft genome and undertook a physiological characterization of TAV2. Strain TAV2 is an autochthonous member of the R. flavipes gut microbiota and groups phylogenetically among diverse Verrucomicrobia from R. flavipes and other termites that are represented by 16S rRNA gene sequences alone. TAV2 is a microaerophile, possessing a high-affinity cbb3-type terminal oxidase-encoding gene and exhibiting an optimum growth rate between 2 and 8% (vol/vol) oxygen. It has the genetic potential to degrade cellulose, an important function within termite guts, but its in vitro substrate utilization spectrum was limited to starch and a few mono- and disaccharides. Growth occurred on nitrogen-free medium, and genomic screening revealed genes for dinitrogenases, heretofore detected in only a few members of the Verrucomicrobia. This represents the first (i) characterization of a verrucomicrobial species from the termite gut, (ii) report of nif and anf genes in a nonacidophilic verrucomicrobial species, and (iii) description of a microaerophilic genotype and phenotype in this phylum of bacteria. The genetic and physiological distinctiveness of TAV2 supports its recognition as the type strain of a new genus and species, for which the name Diplosphaera colitermitum gen. nov., sp. nov., is proposed.
机译：先前，我们报道了从白蚁网状黄褐菌的肠道中培养新型Verrucomicrobia，包括菌株TAV2（与其最接近的栽培代表Opitutus terreae PB90-1的93％16S rRNA基因相同）。为了更好地了解整个Verrucomicrobia，并了解Verrucomicrobia在白蚁肠道生态系统中的作用，我们分析了基因组草图并对其TAV2进行了生理表征。 TAV2菌株是黄褐线虫肠道菌群的自生成员，在黄褐线虫和其他仅由16S rRNA基因序列代表的白蚁的多种疣微生物中，系统发育上属于群。 TAV2是一种微需氧菌，具有高亲和力的cbb3型末端氧化酶编码基因，并具有2至8％（vol / vol）氧气的最佳生长速率。它具有降解纤维素的遗传潜力，这是白蚁内脏中的重要功能，但其体外底物利用范围仅限于淀粉以及一些单糖和双糖。生长在无氮培养基上进行，并且基因组筛选揭示了二氮合酶的基因，迄今为止仅在微疣微生物的少数成员中检测到。这代表了第一个（i）白蚁肠道中的疣状菌种的特征，（ii）非嗜酸性疣状菌种中nif和anf基因的报道以及（iii）该细菌门中的微需氧基因型和表型的描述。 TAV2的遗传和生理独特性支持其被识别为新属和新种的类型菌株，为此命名为Diplosphaera colitermitum gen。十一月，sp。建议。

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