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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Use of Silicate Minerals for pH Control during Reductive Dechlorination of Chloroethenes in Batch Cultures of Different Microbial Consortia
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Use of Silicate Minerals for pH Control during Reductive Dechlorination of Chloroethenes in Batch Cultures of Different Microbial Consortia

机译：硅酸盐矿物在不同微生物群落分批培养物中氯丁酮还原脱氯过程中用于控制pH的用途

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In chloroethene-contaminated sites undergoing in situ bioremediation, groundwater acidification is a frequent problem in the source zone, and buffering strategies have to be implemented to maintain the pH in the neutral range. An alternative to conventional soluble buffers is silicate mineral particles as a long-term source of alkalinity. In previous studies, the buffering potentials of these minerals have been evaluated based on abiotic dissolution tests and geochemical modeling. In the present study, the buffering potentials of four silicate minerals (andradite, diopside, fayalite, and forsterite) were tested in batch cultures amended with tetrachloroethene (PCE) and inoculated with different organohalide-respiring consortia. Another objective of this study was to determine the influence of pH on the different steps of PCE dechlorination. The consortia showed significant differences in sensitivities toward acidic pH for the different dechlorination steps. Molecular analysis indicated that Dehalococcoides spp. that were present in all consortia were the most pH-sensitive organohalide-respiring guild members compared to Sulfurospirillum spp. and Dehalobacter spp. In batch cultures with silicate mineral particles as pH-buffering agents, all four minerals tested were able to maintain the pH in the appropriate range for reductive dechlorination of chloroethenes. However, complete dechlorination to ethene was observed only with forsterite, diopside, and fayalite. Dissolution of andradite increased the redox potential and did not allow dechlorination. With forsterite, diopside, and fayalite, dechlorination to ethene was observed but at much lower rates for the last two dechlorination steps than with the positive control. This indicated an inhibition effect of silicate minerals and/or their dissolution products on reductive dechlorination of cis-dichloroethene and vinyl chloride. Hence, despite the proven pH-buffering potential of silicate minerals, compatibility with the bacterial community involved in in situ bioremediation has to be carefully evaluated prior to their use for pH control at a specific site.
机译：在进行原位生物修复的氯乙烯污染场地中，地下水酸化是源区的常见问题，必须实施缓冲策略以将pH值保持在中性范围内。常规可溶性缓冲剂的替代方法是作为长期碱源的硅酸盐矿物颗粒。在以前的研究中，已经基于非生物溶解测试和地球化学模型评估了这些矿物质的缓冲潜力。在本研究中，在用四氯乙烯（PCE）修正的分批培养物中测试了四种硅酸盐矿物（闪锌矿，透辉石，铁橄榄石和镁橄榄石）的缓冲潜力，并接种了不同的吸入有机卤化物的财团。这项研究的另一个目的是确定pH对PCE脱氯不同步骤的影响。对于不同的脱氯步骤，该财团在对酸性pH的敏感性方面显示出显着差异。分子分析表明，Dehalococcoides spp。与Sulfurospirillum spp相比，所有财团中存在的对pH值最敏感的有机卤化物行会成员。和Dehalobacter spp。在使用硅酸盐矿物颗粒作为pH缓冲剂的分批培养中，测试的所有四种矿物均能够将pH维持在适当的范围内，以进行氯乙烯的还原性脱氯。但是，仅使用镁橄榄石，透辉石和铁橄榄石才能观察到完全脱氯为乙烯。溶解和辐射增加了氧化还原电位，并且不允许脱氯。对于镁橄榄石，透辉石和铁橄榄石，可以观察到脱氯成乙烯，但在最后两个脱氯步骤中的脱氯速率比阳性对照要低得多。这表明硅酸盐矿物和/或其溶解产物对顺式二氯乙烯和氯乙烯的还原脱氯具有抑制作用。因此，尽管已证明硅酸盐矿物具有pH缓冲能力，但在将其用于特定位置的pH控制之前，必须仔细评估与原位生物修复中涉及的细菌群落的相容性。

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