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首页> 美国卫生研究院文献>Antimicrobial Agents and Chemotherapy >BRO beta-lactamases of Branhamella catarrhalis and Moraxella subgenus Moraxella including evidence for chromosomal beta-lactamase transfer by conjugation in B. catarrhalis M. nonliquefaciens and M. lacunata.
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BRO beta-lactamases of Branhamella catarrhalis and Moraxella subgenus Moraxella including evidence for chromosomal beta-lactamase transfer by conjugation in B. catarrhalis M. nonliquefaciens and M. lacunata.

机译：卡他氏杆菌和莫拉氏菌莫拉氏菌的BROβ-内酰胺酶包括通过卡他氏菌非液化性莫拉氏菌和漆膜分枝杆菌结合产生的染色体β-内酰胺酶的证据。

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摘要

Two closely related beta-lactamases, BRO-1 and BRO-2 (formerly called Ravasio and 1908), are found in Moraxella (Branhamella) catarrhalis. We screened strains of B. catarrhalis recovered in the United States since 1952 and identified the first beta-lactamase-positive isolate in August 1976. The prevalence of the enzymes among 394 clinical isolates from one Texas hospital has averaged 75% since testing began in 1983. Screening of isolates of Moraxella subgenus Moraxella revealed the BRO enzymes in two other human respiratory tract species, M. lacunata and M. nonliquefaciens, beginning in 1978. A different beta-lactamase with a pI of 6.4 predominated in other species of subgenus Moraxella. BRO-2 had a different isoelectric focusing pattern and was produced in lesser amounts than BRO-1, but the two enzymes were indistinguishable by substrate or inhibitor profile. BRO enzymes from B. catarrhalis, M. nonliquefaciens, and M. lacunata could be transferred by conjugation and, for B. catarrhalis, also by transformation to B. catarrhalis. Plasmid bands were demonstrated in 90% of M. nonliquefaciens and in one previously reported strain of B. catarrhalis, but no change in plasmid profiles was seen in beta-lactamase-positive recombinants, supporting previous studies that suggested the beta-lactamase genes are chromosomal.
机译：在卡他莫拉氏菌（Branhamella）中发现了两种密切相关的β-内酰胺酶BRO-1和BRO-2（以前称为Ravasio和1908）。我们筛选了自1952年以来在美国回收的卡他芽孢杆菌菌株，并于1976年8月鉴定了第一个β-内酰胺酶阳性分离株。自1983年开始测试以来，得克萨斯州一家医院的394株临床分离株中酶的流行率平均为75％莫拉氏菌亚种的分离株的筛选揭示了从1978年开始的另外两个人类呼吸道物种，M。lacunata和M. nonliquefaciens中的BRO酶。pI为6.4的另一种β-内酰胺酶在其他莫拉氏菌亚种中占主导地位。 BRO-2具有不同的等电聚焦模式，并且产生的量少于BRO-1，但是两种酶在底物或抑制剂谱上无法区分。来自粘膜芽孢杆菌，非液化分枝杆菌和漆膜分枝杆菌的BRO酶可以通过缀合转移，对于粘膜芽孢杆菌，也可以通过转化为粘膜芽孢杆菌来转移。在90％的非液化分枝杆菌和一种先前报道的卡他芽孢杆菌菌株中均显示了质粒条带，但在β-内酰胺酶阳性重组体中未观察到质粒谱的变化，支持先前的研究表明β-内酰胺酶基因为染色体。

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