Use of a Surface Plasmon Resonance Method To Investigate Antibiotic and Plasma Protein Interactions

机译：使用表面等离振子共振方法研究抗生素与血浆蛋白的相互作用

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The pharmacologic effect of an antibiotic is directly related to its unbound concentration at the site of infection. Most commercial antibiotics have been selected in part for their low propensity to interact with serum proteins. These nonspecific interactions are classically evaluated by measuring the MIC in the presence of serum. As higher-throughput technologies tend to lose information, surface plasmon resonance (SPR) is emerging as an informative medium-throughput technology for hit validation. Here we show that SPR is a useful automatic tool for quantification of the interaction of model antibiotics with serum proteins and that it delivers precise real-time kinetic data on this critical parameter.

机译：抗生素的药理作用直接与其在感染部位的未结合浓度有关。选择大多数商业抗生素的部分原因是它们与血清蛋白相互作用的倾向很低。这些非特异性相互作用通常通过在血清存在下测量MIC来评估。随着高通量技术趋向于丢失信息，表面等离振子共振（SPR）逐渐成为一种有用的中等通量技术，可用于命中验证。在这里，我们显示SPR是用于定量模型抗生素与血清蛋白相互作用的有用自动工具，并且它可针对此关键参数提供精确的实时动力学数据。

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期刊名称 Antimicrobial Agents and Chemotherapy
作者
Françoise Banères-Roquet; Maxime Gualtieri; Philippe Villain-Guillot; Martine Pugnière; Jean-Paul Leonetti;
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作者单位

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年(卷),期 2009(53),4
年度 2009
页码 1528–1531
总页数 4
原文格式 PDF
正文语种
中图分类药学;微生物学;
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专利

1. Use of a surface plasmon resonance method to investigate antibiotic and plasma protein interactions. [J] . Baneres Roquet F, Gualtieri M, Villain Guillot P Antimicrobial agents and chemotherapy. . 2009,第4期

机译：使用表面等离子体共振方法研究抗生素和血浆蛋白的相互作用。
2. HEPARINIZATION OF GAS PLASMA-MODIFIED POLYSTYRENE SURFACES AND THE INTERACTIONS OF THESE SURFACES WITH PROTEINS STUDIED WITH SURFACE PLASMON RESONANCE [J] . Vandelden CJ., Kooyman RPH., Engbers GHM., Biomaterials . 1997,第12期

机译：气体等离子体改性的聚苯乙烯表面的肝素化及这些表面与表面等离子体共振产生的蛋白质的相互作用
3. HEPARINIZATION OF GAS PLASMA-MODIFIED POLYSTYRENE SURFACES AND THE INTERACTIONS OF THESE SURFACES WITH PROTEINS STUDIED WITH SURFACE PLASMON RESONANCE [J] . Vandelden CJ., Kooyman RPH., Engbers GHM., Biomaterials . 1997,第12期

机译：气体等离子体改性的聚苯乙烯表面的肝素化及这些表面与表面等离子体共振产生的蛋白质的相互作用
4. High-Throughput Study of Protein-Surface Interactions Using a Surface Plasmon Resonance Imaging Apparatus [C] . Yusuke Arima, Rika Ishii, Isao Hirata, Proteins at interfaces III: State of the art . 2012

机译：使用表面等离振子共振成像设备的蛋白质-表面相互作用的高通量研究
5. Surface plasmon resonance imaging studies of protein-carbohydrate interactions, protein-DNA interactions, and chemically induced hairpin formation in DNA monolayers. [D] . Smith, Emily Ann. 2003

机译：表面等离振子共振成像研究了蛋白质-碳水化合物相互作用，蛋白质-DNA相互作用以及化学诱导的DNA单层发夹形成。
6. Kinetic and thermodynamic study of beta-Boswellic acid interaction with Tau protein investigated by surface plasmon resonance and molecular modeling methods [O] . Hossein Haghaei, Seyed Rafie Aref Hosseini, Somaieh Soltani, 2020

机译：表面等离子体共振和分子建模方法研究β-乳香酸与Tau蛋白相互作用的动力学和热力学研究
7. Use of a Surface Plasmon Resonance Method To Investigate Antibiotic and Plasma Protein Interactions ▿ [O] . Banères-Roquet, Françoise, Gualtieri, Maxime, Villain-Guillot, Philippe, 2009

机译：使用表面等离子体共振方法研究抗生素和血浆蛋白的相互作用
8. Novel Protein-Protein Interactions of the Yersinia pestis Type III secretion System Elucidated With a Matrix Analysis by Surface Plasmon Resonance and Mass Spectrometry [R] . Swietnicki, W. , O'Brien, S. , Holman, K. , 2004

机译：通过表面等离子体共振和质谱法进行基质分析阐明的鼠疫耶尔森氏菌III型分泌系统的新蛋白质 - 蛋白质相互作用

Use of a Surface Plasmon Resonance Method To Investigate Antibiotic and Plasma Protein Interactions

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