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首页> 美国卫生研究院文献>Annals of the Rheumatic Diseases >Identification of the advanced glycation end products N -carboxymethyllysine in the synovial tissue of patients with rheumatoid arthritis
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Identification of the advanced glycation end products N -carboxymethyllysine in the synovial tissue of patients with rheumatoid arthritis

机译：类风湿关节炎患者滑膜组织中晚期糖基化终产物N-羧甲基赖氨酸的鉴定

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Background: Generation of advanced glycation end products (AGEs) is an inevitable process in vivo and can be accelerated under pathological conditions such as oxidative stress. In serum and synovial fluid of patients with rheumatoid arthritis (RA) raised AGE levels have been found. Objective: To determine the presence of N -carboxymethyllysine (CML; marker of oxidative stress) in RA synovial tissue by immunohistology. Methods: Frozen synovial tissue samples from 10 patients with RA and eight controls (four patients without joint disease and four patients with osteoarthritis (OA)) were treated with rabbit-anti-CML-IgG and goat-antirabbit-IgG. Immunostaining was visualised by streptavidine-alkaline phosphatase (chromogen fuchsin). Cell differentiation was performed with antibodies against CD68, CD45RO, and CD20. Results: CML was detected in the synovial lining, sublining, and endothelium in 10/10 RA and 4/4 OA synovial specimens. In RA some macrophages (CD68+) and T cells (CD45RO+) showed positive immunostaining for CML, whereas B cells were negative. Staining in OA synovial sublining was weak compared with RA. Conclusions: CML was detected for the first time in RA and OA synovial tissue. Different patterns of immunostaining in RA and OA and the presence of CML on macrophages and T cells, suggest a role for CML in the pathogenesis of RA. This might be due to presentation of new epitopes which can maintain or even trigger an autoimmune response.
机译：背景：高级糖基化终产物（AGEs）的产生是体内不可避免的过程，并且可以在诸如氧化应激等病理条件下加速。在类风湿关节炎（RA）患者的血清和滑液中，已发现AGE水平升高。目的：通过免疫组织学方法确定RA滑膜组织中N-羧甲基赖氨酸（CML；氧化应激标记）的存在。方法：用兔抗CML-IgG和山羊抗兔-IgG处理10例RA患者和8例对照（4例无关节病和4例骨关节炎（OA））的冷冻滑膜组织样品。免疫染色通过链霉亲和素碱性磷酸酶（发色品红）观察。用针对CD68，CD45RO和CD20的抗体进行细胞分化。结果：在10/10 RA和4/4 OA滑膜标本的滑膜衬里，亚衬层和内皮中检测到CML。在RA中，某些巨噬细胞（CD68 +）和T细胞（CD45RO +）对CML表现出阳性免疫染色，而B细胞则呈阴性。与RA相比，OA滑膜下层的染色较弱。结论：RA和OA滑膜组织中首次检测到CML。 RA和OA中免疫染色的不同模式以及巨噬细胞和T细胞上存在CML，表明CML在RA发病机理中的作用。这可能是由于出现了新的表位，它们可以维持甚至触发自身免疫反应。

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