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Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis

机译：SYBR Green1单管多重实时PCR和高分辨率熔解分析法检测α-地中海贫血-1东南亚和泰国型缺失和β-地中海贫血3.5-kb缺失

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BackgroundPrevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia.

机译：背景地中海贫血的预防和控制要求对可能怀有严重地中海贫血胎儿风险的携带者夫妇进行简单，快速和准确的筛查测试。

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期刊名称 Annals of Laboratory Medicine
作者
Sakorn Pornprasert; Thanatcha Wiengkum; Sarinee Srithep; Isarapong Chainoi; Panthong Singboottra; Sanchai Wongwiwatthananukit;
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作者单位

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年(卷),期 2011(31),3
年度 2011
页码 138–142
总页数 5
原文格式 PDF
正文语种
中图分类情报学;
关键词
Multiplex real-time PCR SYBR Green1 α-thalassemia-1 β-thalassemia 3.5-kb deletion High-resolution melting analysis;

机译：多重实时PCR;SYBR Green1;α-地中海贫血1;β-地中海贫血3.5kb缺失;高分辨率熔解分析;

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专利

1. Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis [J] . Annals of laboratory medicine. . 2011,第3期

机译：SYBR Green1单管多重实时PCR和高分辨率熔解分析法检测α-地中海贫血-1东南亚和泰国型缺失和β-地中海贫血3.5-kb缺失
2. Rapid diagnosis of the alpha-thalassemia-1 Southeast Asian type deletion using a single tube real-time SYBR-polymerase chain reaction combined with dissociation curve analysis. [J] . Liu JZ, Xiao B, Wang Q Hemoglobin: International Journal for Hemoglobin Research . 2009,第6期

机译：使用单管实时SYBR-聚合酶链反应结合解离曲线分析快速诊断α-地中海贫血1东南亚型缺失。
3. Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the alpha-thalassemia-1 Southeast Asian type deletion: application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis. [J] . Pornprasert S, Sukunthamala K, Sacome J, Hemoglobin: International Journal for Hemoglobin Research . 2008,第4期

机译：实时SYBR聚合酶链反应周期阈值分析，用于诊断α-地中海贫血1东南亚型缺失：在Hb Bart胎儿积水的携带者筛查和产前诊断中的应用。
4. Real-Time PCR and melting curve analysis for rapid detection and identification of beer spoilage microorganisms [C] . Youichi Tsuchiya, Yasukazu Nakakita, Yasunobu Nara, EBC congress;Dublin 2003 . 2003

机译：实时PCR和解链曲线分析可快速检测和鉴定啤酒腐败菌
5. Simultaneous detection and discrimination of Echinococcus granulosus and Echinococcus multilocularis using real-time polymerase chain reaction and high-resolution melting analysis [D] . Olson, Kathryn Mary 2016

机译：实时聚合酶链反应和高分辨率熔解分析同时检测和鉴别细粒棘球and和多眼棘球oc
6. Low fetal hemoglobin rates in patients carrying Thai (δβ)0-deletion and Turkish (δβ)0-deletion/inversion strengthen the hypothesis that the 5′δ BCL11A binding site plays a major role in its fetal hemoglobin inhibitory regulation. Response to The 12.6 kb-deletion in the β-globin gene cluster is the known Thai/Vietnamese (δβ)0-thalassemia commonly found in Southeast Asia [O] . Elyes Slim Ghedira, Serge Pissard 2013

机译：携带Thai（δβ）0缺失和土耳其语（δβ）0缺失/倒置的患者的胎儿血红蛋白率低强化了以下假设：5δBCL11A结合位点在其胎儿血红蛋白抑制调节中起主要作用。对β-珠蛋白基因簇中12.6 kb的缺失是东南亚常见的已知泰国/越南（δβ）0地中海贫血的回应
7. Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis [O] . Pornprasert, Sakorn, Wiengkum, Thanatcha, Srithep, Sarinee, 2011

机译：SYBR Green1单管多重实时PCR和高分辨率熔解分析法检测α-地中海贫血-1东南亚和泰国型缺失和β-地中海贫血3.5-kb缺失
8. Multiplex PCR Analysis of In vivo-Arising Deletion Mutations in the 'hprt' Geneof Human T-Lymphocytes [R] . Fuscoe, J. C., Zimmerman, L. J., Harrington-Brock, K., 1994

机译：人类T淋巴细胞'hprt'基因体内缺失突变的多重pCR分析

Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis

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