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首页> 美国卫生研究院文献>Annals of Botany >Genetic Fingerprinting of Germplasm Accessions as an Aid for Species Conservation: A Case Study with Borderea chouardii (Dioscoreaceae) One of the Most Critically Endangered Iberian Plants
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Genetic Fingerprinting of Germplasm Accessions as an Aid for Species Conservation: A Case Study with Borderea chouardii (Dioscoreaceae) One of the Most Critically Endangered Iberian Plants

机译：种质种质的遗传指纹图谱作为物种保护的辅助手段：以濒危物种伊比利亚最严重的植物之一博古纲（Dioscoreaceae）为例

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• Background and Aims Molecular markers have changed previous expectations about germplasm collections of endangered plants, as new perspectives aim at holding a significant representation of all the genetic diversity in the studied species to accomplish further conservation initiatives successfully. Borderea chouardii is a critically endangered allotetraploid dioecious member of Dioscoreaceae, known from a single population in the Iberian pre-Pyrenees. This population was reported to be highly structured into two genetically distinct groups of individuals corresponding to their spatial separation along the vertical cliff where it grows. In 1999, the Spanish Government of Aragón launched the first conservation programme for the ex situ preservation of this species, and since then a seed collection has been conserved at the Germplasm Bank of the Universidad Politécnica de Madrid. However, as some seed samples had not been labelled clearly at the time of collection, their origin was uncertain.• Methods Genetic variation in germplasm accessions of B. chouardii was investigated using microsatellite (simple sequence repeat; SSR) markers.• Key Results The 17 primer pairs used detected 62 SSR alleles in the 46 samples analysed from five different germplasm stocks. Eight alleles scored from the wild population were not detected in the germplasm samples analysed. The relatedness of the germplasm samples to the wild subpopulations through neighbour-joining clustering, principal coordinates analysis (PCO) and assignment tests revealed a biased higher representation of the genetic diversity of the lower cliff (43 samples) subpopulation than that of the upper cliff (three samples).• Conclusions The collection of additional samples from the upper cliff is recommended to achieve a better representation of the genetic diversity of this subpopulation. It is also recommended that these stocks should be managed separately according to their distinct microspatial origin in order to preserve the genetic substructuring of the wild population.
机译：•背景和目的分子标记改变了以前对濒危植物种质收集的期望，因为新的观点旨在掌握所研究物种中所有遗传多样性的重要代表，从而成功地完成进一步的保护计划。 Borderea chouardii是薯Di科的极度濒危的异源四倍体雌雄异株成员，从伊比利亚比利牛斯​​山前的一个种群中得知。据报道，该种群高度结构化为两个遗传上不同的个体群，这与他们沿其生长的垂直悬崖的空间分隔相对应。 1999年，西班牙阿拉贡政府发起了第一个异地保存该物种的保护计划，此后，种子收集一直保存在马德里政治大学的种质库中。但是，由于一些种子样品在收集时尚未被明确标记，因此其来源尚不确定。•方法使用微卫星（简单重复序列； SSR）标记研究了霍乱芽孢杆菌种质的遗传变异。•主要结果在从五种不同种质资源中分析的46份样品中，使用17对引物检测出62个SSR等位基因。在分析的种质样本中未检测到来自野生种群的八个等位基因。通过近邻聚类，主坐标分析（PCO）和分配测试，种质样品与野生亚种群的相关性显示，下悬崖（43个样品）亚种群的遗传多样性比上悬崖（结论•建议从悬崖上部收集更多样本，以更好地表示该亚群的遗传多样性。还建议应根据其不同的微空间来源对这些种群进行单独管理，以保留野生种群的遗传结构。

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