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Molecular Taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and Malaria Epidemiology in Southern Amazonian Peru

机译:秘鲁南部亚马逊南部按蚊(Nyssorhynchus)benarrochi(Diptera:Culicidae)的分子分类学和疟疾流行病学

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摘要

Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either Anopheles (Nyssorhynchus) benarrochi B or An. (Nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as An. benarrochi B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian An. benarrochi B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA COI BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2+COI detected a single clade with very high support for all An. benarrochi B except one individual from Lupuna that was excluded. No samples were positive for Plasmodium by CytB-PCR.
机译:2011年,秘鲁秘鲁马德雷迪奥斯部门的圣罗莎和圣佩德罗疟疾流行地区的人类着陆捕获物,香农和CDC诱捕器收集了按蚊标本。多数标本是按蚊(Nyssorhynchus)benarrochi B或An。 (Nys。)rangeli,通过聚合酶链反应-限制性片段长度多态性-内部转录间隔区2(PCR-RFLP-ITS2),以及对于选定的个体,ITS2序列证实。秘鲁北部亚马逊北部洛雷托省Lupuna的一些标本也被确定为An。 benarrochi B.使用ITS2序列的统计简约网络确认所有秘鲁人。分析的benarrochi B与来自哥伦比亚南部普图马约的GenBank中的benarrochi B相同。尽管在秘鲁北部和南部序列之间存在多个突变步骤,但使用统计简约网络连接了来自所有三个秘鲁地区的标本的mtDNA COI BOLD区域序列。对秘鲁的ITS2 + COI序列进行贝叶斯推断,发现单个进化枝对所有An都具有很高的支持度。 benarrochi B,但来自Lupuna的一名个人被排除在外。通过CytB-PCR没有发现疟原虫阳性。

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