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Chromosomal Rearrangement Features of Yersinia pestis Strains from Natural Plague Foci in China

机译:中国天然疫源性鼠疫耶尔森菌菌株的染色体重排特征

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摘要

The Yersinia pestis chromosome contains a large variety and number of insert sequences that have resulted in frequent chromosome rearrangement events. To identify the chromosomal rearrangement features of Y. pestis strains from five typical plague foci in China and study spontaneous DNA rearrangements potentially stabilized in certain lineages of Y. pestis genomes, we examined the linking mode of locally collinear blocks (LCBs) in 30 Y. pestis strains by a polymerase chain reaction-based method. Our results suggest most strains have relatively stable chromosomal arrangement patterns, and these rearrangement characteristics also have a very close relationship with the geographical origin. In addition, some LCB linking modes are only present in specific strains. We conclude Y. pestis chromosome rearrangement patterns may reflect the genetic features of specific geographical areas and can be applied to distinguish Y. pestis isolates; furthermore, most of the rearrangement events are stable in certain lineages of Y. pestis genomes.
机译:鼠疫耶尔森氏菌染色体包含种类繁多和数量众多的插入序列,这些插入序列导致频繁的染色体重排事件。为了确定中国5个典型鼠疫疫源地鼠疫耶尔森氏菌的染色体重排特征,并研究在鼠疫耶尔森菌某些基因系中可能稳定的自发DNA重排,我们研究了30 Y中局部共线区(LCB)的连接方式。鼠疫菌株通过聚合酶链反应为基础的方法。我们的结果表明,大多数菌株具有相对稳定的染色体排列方式,并且这些重排特征也与地理起源有着非常密切的关系。此外,某些LCB连接模式仅在特定菌株中存在。我们得出结论:鼠疫耶尔森氏菌染色体重排模式可能反映了特定地理区域的遗传特征,可用于区分鼠疫耶尔森氏菌。此外,大多数重排事件在鼠疫耶尔森氏菌基因组的某些谱系中是稳定的。

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