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首页> 美国卫生研究院文献>American Journal of Translational Research >Extracorporeal shock wave therapy ameliorates cyclophosphamide-induced rat acute interstitial cystitis though inhibiting inflammation and oxidative stress-in vitro and in vivo experiment studies
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Extracorporeal shock wave therapy ameliorates cyclophosphamide-induced rat acute interstitial cystitis though inhibiting inflammation and oxidative stress-in vitro and in vivo experiment studies

机译：体外冲击波疗法通过在体外和体内实验研究中抑制炎症和氧化应激改善了环磷酰胺诱导的大鼠急性间质性膀胱炎

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Background: We investigated whether extracorporeal shock wave (ECSW) therapy can attenuate cyclophosphamide (CYP)-induced acute interstitial cystitis (AIC) in rats. Methods and Results: Eighteen male-adult Sprague-Dawley rats were equally divided into group 1 (sham control), group 2 (AIC induced by 150 mg/kg CYP by intra-peritoneal injection) and group 3 (AIC + ECSW 200 impulses at 0.11 mJ/mm2 to the urinary bladder at 3 and 24 h after CYP treatment). Smooth-muscle cells co-culture with menadione (25 µM) with and without ECSW treatment was performed. Western-blot results demonstrated that ECSW significant attenuated oxidative stress and inflammatory reactions in this in-vitro studies (all p < 0.001). 24-hour urine amount and microscopic findings of red-blood-cell count (i.e., hematuria) were higher in group 2 than in groups 1 and 3, and significantly higher in group 3 than in group 1 (all p < 0.001). The urine levels of albumin and interleukin-6 showed an identical pattern of hematuria among all three groups (all p < 0.001). The cellular and mRNA expressions of macrophage migration inhibitory factor (MIF)+, CD74+, CD68+, substance p+, and Cox-2+ cells in the bladder tissue exhibited an identical pattern of hematuria among all groups (all p < 0.0001). The integrity of epithelial layer and collagen-deposition area as stained by Sirius red displayed an opposite pattern of hematuria among the three groups (p < 0.0001). The protein expression of IL-12, iNOS, TNF-α, NF-κB, MMP-9, NOX-1, NOX-2, RANTES, and Oxyblot displayed an identical pattern of hematuria among all groups (all p < 0.01). Conclusion: ECSW therapy markedly attenuated CYP-induced AIC through inhibitions of the inflammation and oxidative stress.
机译：背景：我们调查了体外冲击波（ECSW）治疗是否可以减轻环磷酰胺（CYP）诱导的大鼠急性间质性膀胱炎（AIC）。方法和结果：将18只雄性成年Sprague-Dawley大鼠平均分为第1组（假对照组），第2组（腹膜内注射150 mg / kg CYP诱导的AIC）和第3组（AIC + ECSW 200脉冲） CYP治疗后3和24小时向膀胱移入0.11 mJ / mm 2 。进行带有和不带有ECSW处理的与甲萘醌（25 µM）共培养的平滑肌细胞。 Western印迹结果表明，在这项体外研究中ECSW显着减轻了氧化应激和炎症反应（所有p <0.001）。第2组的24小时尿液量和显微镜下的红细胞计数（即血尿）高于第1组和第3组，而第3组显着高于第1组（所有p <0.001）。在所有三组中，白蛋白和白细胞介素6的尿液水平显示出相同的血尿模式（所有p <0.001）。膀胱组织中巨噬细胞迁移抑制因子（MIF）+，CD74 +，CD68 +，物质p +和Cox-2 +细胞的细胞和mRNA表达在所有组中均表现出相同的血尿模式（所有p <0.0001）。天狼星红染色的上皮层和胶原沉积区域的完整性在三组中表现出相反的血尿模式（p <0.0001）。 IL-12，iNOS，TNF-α，NF-κB，MMP-9，NOX-1，NOX-2，RANTES和Oxyblot的蛋白表达在所有组中均表现出相同的血尿模式（所有p <0.01）。结论：ECSW疗法通过抑制炎症和氧化应激而显着减弱CYP诱导的AIC。

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