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首页> 美国卫生研究院文献>Journal of Nucleic Acids >Systemic Identification of Hevea brasiliensis EST-SSR Markers and Primer Screening
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Systemic Identification of Hevea brasiliensis EST-SSR Markers and Primer Screening

机译：巴西橡胶树EST-SSR标记的系统鉴定和引物筛选

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摘要

This research aimed to systematically identify and preliminarily validate the Hevea brasiliensis expressed sequence tag (EST) information using Simple Sequence Repeat (SSR) and provide evidence for further development of SSR molecular marker. The definition of general SSR features of Hevea EST splicing sequences and development of SSR primers founded the basis of diversity analysis and variety identification for Hevea tree resource. 1134 SSR loci were identified in the EST splicing sequence and distributed in 840 Unigene. The occurrence rate of SSR loci was 23.9%, and the average distribution distance of EST-SSR was 2.59 kb. The major repeat type was mononucleotide repeat motif, which accounted for 38.89%, while the corresponding value was 36.95% for dinucleotide repeat motif and 18.17% for trinucleotide repeat motif; the proportion of other motifs was only 5.99%. The superior repeat motifs for mononucleotide, dinucleotide, and trinucleotide were A/T, AG/CT, and AAG/CTT, respectively. 739 pair of primers were designed for 1134 SSR loci. PCR amplification was performed on Hevea Reyan5-11, Reyan87-6-47, and PR107, and 180 pairs of primers were selected which were able to amplify polymorphism bands.
机译：这项研究旨在系统鉴定和初步验证巴西橡胶树表达序列标签（EST）信息的使用简单序列重复（SSR），并为进一步开发SSR分子标记提供证据。橡胶树EST剪接序列的一般SSR特征的定义和SSR引物的开发奠定了橡胶树资源多样性分析和品种鉴定的基础。在EST剪接序列中鉴定出1134个SSR基因座，并分布在840 Unigene中。 SSR基因座的发生率为23.9％，EST-SSR的平均分布距离为2.59 kb。主要重复类型为单核苷酸重复基序，占38.89％，二核苷酸重复基序为36.95％，三核苷酸重复基序为18.17％。其他图案的比例仅为5.99％。单核苷酸，二核苷酸和三核苷酸的优良重复基序分别为A / T，AG / CT和AAG / CTT。为1134个SSR基因座设计了739对引物。在三叶胶Reyan5-11，Reyan87-6-47和PR107上进行PCR扩增，选择了180对引物，它们能够扩增多态性条带。

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